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For this purpose, C57BL\/6 mice were whole\u2010body exposed to three cigarettes daily, 7 days\/week, for 2 or 4 months by a specific rodent ventilator. Mice were then superovulated and oocytes collected. MII oocytes pools obtained by single animals were deprived of cumulus cells and used to extract total RNA including miRNAs. TaqMan\u2122 Rodent MicroRNA A Array v2.0 was used to analyze the miRNAs expression profile. The biological functions and the functional networks of the identified up\u2010 and downregulated miRNAs were analyzed by ingenuity pathway analysis software. The gene expression of deregulated\u2010miRNAs targets was evaluated. For the first time, the global miRNAs changes in mouse oocyte in response to cigarette smoke exposure were disclosed. Our results revealed significant modulation of miRNAs mainly involved in inflammatory processes, cellular proliferation, and apoptosis. miRNAs expression was altered in a time\u2010dependent manner. Smoke exposure induced an early downregulation of Dicer1. Transcriptional alterations of the modulated miRNAs major targets, estrogen receptor 1, peroxisome proliferator\u2010activated receptor\u2010alpha, and tumor protein 53, as well as that of other key regulatory genes, were evidenced. Cigarette smoke represents a stimulus able to alter miRNAs pattern in mouse oocyte. This study increases our understanding of the ovarian toxicity profile of cigarette smoke, and open new roads toward the identification of biomarkers of oocyte toxicity and dysregulation.<\/jats:p>","DOI":"10.1002\/mrd.23267","type":"journal-article","created":{"date-parts":[[2019,9,12]],"date-time":"2019-09-12T04:08:46Z","timestamp":1568261326000},"page":"1741-1757","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Whole\u2010body exposure to cigarette smoke alters oocyte miRNAs expression in C57BL\/6 mice"],"prefix":"10.1002","volume":"86","author":[{"given":"Maria Cristina","family":"Budani","sequence":"first","affiliation":[{"name":"Department of Medicine and Aging Sciences University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"D'Aurora","sequence":"additional","affiliation":[{"name":"Department of Psychological, Health and Territorial Sciences (DISPUTer), Laboratory of Molecular Genetics, School of Medicine and Health Sciences University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"},{"name":"Center of Excellence on Aging and Translational Medicine \u2010 (CeSI\u2010MeT) University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liborio","family":"Stuppia","sequence":"additional","affiliation":[{"name":"Department of Psychological, Health and Territorial Sciences (DISPUTer), Laboratory of Molecular Genetics, School of Medicine and Health Sciences University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"},{"name":"Center of Excellence on Aging and Translational Medicine \u2010 (CeSI\u2010MeT) University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Gatta","sequence":"additional","affiliation":[{"name":"Department of Psychological, Health and Territorial Sciences (DISPUTer), Laboratory of Molecular Genetics, School of Medicine and Health Sciences University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"},{"name":"Center of Excellence on Aging and Translational Medicine \u2010 (CeSI\u2010MeT) University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3030-5230","authenticated-orcid":false,"given":"Gian Mario","family":"Tiboni","sequence":"additional","affiliation":[{"name":"Department of Medical, Oral and Biotechnological Sciences University \u201cG. d'Annunzio\u201d Chieti\u2010Pescara  Chieti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,11]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dex320"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.07.031"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)49517-8"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.120125mz"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.2000.9006"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph16020263"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199417000132"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2018.06.001"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2017.06.184"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.09.014"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2001.281.2.L412"},{"key":"e_1_2_6_1_14_1","volume-title":"How tobacco smoke causes disease: The biology and behavioral basis for smoking\u2010attributable disease: A report of the surgeon general","author":"Centers for Disease Control and Prevention; 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