{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T15:28:42Z","timestamp":1792855722710,"version":"4.5.0"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,11,17]],"date-time":"2019-11-17T00:00:00Z","timestamp":1573948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["Research on Innovation Areas\/17H05845, 19H04868"],"award-info":[{"award-number":["Research on Innovation Areas\/17H05845, 19H04868"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["\u00a0JSPS Research Fellow\/19J12912"],"award-info":[{"award-number":["\u00a0JSPS Research Fellow\/19J12912"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K14742"],"award-info":[{"award-number":["16K14742"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Polyploidization has played a major role in the long\u2010term diversification and evolutionary success of angiosperms. Triploid formation among diploid plants, which is generally considered to be achieved by fertilization of an unreduced gamete with a reduced one, has been accepted as a means of polyploid production. In addition, it has been supposed that polyspermy also contributes to the triploid formation in maize, wheat, and some orchids; however, such a mechanism has been considered uncommon because reproducing the polyspermic situation and unambiguously investigating developmental profiles of polyspermic zygotes are difficult. To overcome these problems, rice polyspermic zygotes have been successfully produced by electrofusion of an egg cell with two sperm cells, and their developmental profiles have been monitored. The triploid zygotes progress through karyogamy and divide into two\u2010celled embryos via a typical bipolar mitotic division; the two\u2010celled embryos further develop into triploid plants, indicating that polyspermic plant zygotes, unlike those of animals, can develop normally. Furthermore, progenies consisting of triparental genetic materials have been successfully obtained in\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    through the pollination of two different kinds of male parents with a female parent. These different pieces of evidence for development and emergence of polyspermic zygotes in vitro and in planta suggest that polyspermy is a key event in polyploidization and species diversification.\n                  <\/jats:p>","DOI":"10.1002\/mrd.23295","type":"journal-article","created":{"date-parts":[[2019,11,17]],"date-time":"2019-11-17T23:04:25Z","timestamp":1574031865000},"page":"374-379","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Polyspermy in angiosperms: Its contribution to polyploid formation and speciation"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4786-2102","authenticated-orcid":false,"given":"Erika","family":"Toda","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Tokyo Metropolitan University Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6531-210X","authenticated-orcid":false,"given":"Takashi","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Tokyo Metropolitan University Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1201-1120"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.04.041"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02683"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.021345"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00452"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(91)90482-I"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erq371"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2007.02084.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.4825606"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-019-0382-3"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5645"},{"key":"e_1_2_11_13_1","doi-asserted-by":"crossref","first-page":"2867","DOI":"10.1242\/dev.124.15.2867","article-title":"First evidence of a calcium transient in flowering plants at fertilization","volume":"124","author":"Digonnet C.","year":"1997","journal-title":"Development"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.11.041"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.263.5153.1598"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2006357"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.10.059"},{"key":"e_1_2_11_18_1","first-page":"129","article-title":"Sur les anth\u00e9rozoides et la double copulation sexuelle chez les v\u00e9g\u00e9taux angiosperms","volume":"11","author":"Guignard M. 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