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It is crucial to maintain oocytes at the GV stage for a long period to analyze meiotic resumption in vitro. Meiotic resumption can be reversibly inhibited in isolated oocytes by cAMP modulator forskolin, cAMP analog dibutyryl cAMP (dbcAMP), or PDE inhibitors, milrinone (Mil), Cilostazol (CLZ), and 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX). However, these chemicals negatively affect oocyte development and maturation when used independently. Here, we used ICR mice to develop a model that could maintain GV\u2010stage arrest with minimal toxic effects on subsequent oocyte and embryonic development. We identified optimal concentrations of forskolin, dbcAMP, Mil, CLZ, IBMX, and their combinations for inhibiting oocyte meiotic resumption. Adverse effects were assessed according to subsequent development potential, including meiotic resumption after washout, first polar body extrusion, early apoptosis, double\u2010strand DNA breaks, mitochondrial distribution, adenosine triphosphate levels, and embryonic development. Incubation with a combination of 50.0\u2009\u03bcM dbcAMP and 10.0\u2009\u03bcM IBMX efficiently inhibited meiotic resumption in GV\u2010stage oocytes, with low toxicity on subsequent oocyte maturation and embryonic development. This work proposes a novel method with reduced toxicity to effectively arrest and maintain mouse oocytes at the GV stage.<\/jats:p>","DOI":"10.1002\/mrd.23315","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T12:05:58Z","timestamp":1578917158000},"page":"262-273","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Combined use of dbcAMP and IBMX minimizes the damage induced by a long\u2010term artificial meiotic arrest in mouse germinal vesicle oocytes"],"prefix":"10.1002","volume":"87","author":[{"given":"Xue\u2010Chen","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Regulation &amp; Breeding of Grassland Livestock School of Life Sciences Inner Mongolia University Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Han","sequence":"additional","affiliation":[{"name":"State Key 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