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Scrotal insulation (SI) for 48\u2009hr was applied to Brahman (<jats:italic>Bos indicus<\/jats:italic>) bulls. Semen was collected at 3\u2010day intervals from before, until 74 days post\u2010SI. The changes in sperm morphology and motility following SI were comparable to previously reported and differences were detected in measures of sperm chromatin conformation as early as 8 days post\u2010SI. New proteins spots, in the SPP two\u2010dimensional (2\u2010D) gels, were apparent when comparing pre\u2010SI with 74 days post\u2010SI, and SPP identified as associated with mechanisms of cellular repair and protection. Similar trends between 2\u2010D gel and Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH\u2010MS) data was observed, with SWATH\u2010MS able to quantify individual SPP that otherwise were not resolved on 2\u2010D gel. The SPP assessment at peak sperm damage (21\u201324 days) showed a significant difference in 29 SPP (adjusted <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), and identified six proteins with change in abundance in the SI group. In conclusion both spermatozoa and SPP composition of bulls are susceptible to temperature change incurred by SI, and SPP markers for testicular heat insults may be detected.<\/jats:p>","DOI":"10.1002\/mrd.23328","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T12:05:31Z","timestamp":1582286731000},"page":"574-597","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Effects of increased scrotal temperature on semen quality and seminal plasma proteins in Brahman bulls"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2453-1992","authenticated-orcid":false,"given":"Gry Brandt","family":"Boe\u2010Hansen","sequence":"first","affiliation":[{"name":"School of Veterinary Science, The University of Queensland Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Paulo A.","family":"R\u00eago","sequence":"additional","affiliation":[{"name":"Federal Institute of Education, Science and Technology of Cear\u00e1, Boa Viagem Campus Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4895-7437","authenticated-orcid":false,"given":"Nana","family":"Satake","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food Sciences, The University of Queensland St. Lucia QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bronwyn","family":"Venus","sequence":"additional","affiliation":[{"name":"Agri\u2010Science Department of Agriculture Fisheries and Forestry Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1812-3490","authenticated-orcid":false,"given":"Pawel","family":"Sadowski","sequence":"additional","affiliation":[{"name":"Central Analytical Research Facility Queensland University of Technology Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Nouwens","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, The University of Queensland St. Lucia QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutao","family":"Li","sequence":"additional","affiliation":[{"name":"CSIRO Agriculture and Food St. Lucia QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0783-4748","authenticated-orcid":false,"given":"Michael","family":"McGowan","sequence":"additional","affiliation":[{"name":"School of Veterinary Science, The University of Queensland Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065083"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(74)84836-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929866054864292"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20578"},{"issue":"2","key":"e_1_2_8_6_1","first-page":"93","article-title":"The sequential appearance of sperm abnormalities after scrotal insulation or dexamethasone treatment in bulls","volume":"35","author":"Barth A. 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