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AGF was collected by an artificial vagina from five vasectomized rams. Metabolites were identified by gas chromatography\u2010mass spectrometry (GC\/MS) and high\u2010performance liquid chromatography\u2010mass spectrometry (LC\/MS), with the support of Human Metabolome Database, PubChem, LIPID Metabolites, Pathways Strategy databases, and MetaboAnalyst platforms. There were 182 and 190 metabolites detected by GC\/MS and LC\/MS, respectively, with an overlap of one molecule. Lipids and lipid\u2010like molecules were the most abundant class of metabolites in the ram AGF (127 compounds), followed by amino acids, peptides, and analogs(103 metabolites). Considering all GC\/MS and LC\/MS, fructose, glycerol, citric acid, <jats:sc>d<\/jats:sc>\u2010mannitol, <jats:sc>d<\/jats:sc>\u2010glucose, and <jats:sc>l<\/jats:sc>\u2010(+)\u2010lactic acid were the most abundant single metabolites present in the ram AGF. Meaningful pathways associated with AGF metabolites included glycine, serine and threonine metabolism; pantothenate and CoA biosynthesis; galactose metabolism; glutamate metabolism and phenylalanine metabolism, and so forth. In conclusion, the combined use of LC\/MS and GC\/MS was essential for getting a holistic view of the compounds embedded in the ram AGF. Chemical analysis of the accessory sex gland secretion is relevant for understanding sperm function and fertilization.<\/jats:p>","DOI":"10.1002\/mrd.23337","type":"journal-article","created":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T11:16:00Z","timestamp":1584962160000},"page":"409-418","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Untargeted metabolomic profiling of accessory sex gland fluid from Morada Nova rams"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6246-536X","authenticated-orcid":false,"given":"Solange D.","family":"Sousa","sequence":"first","affiliation":[{"name":"Department of Animal Science Federal University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5133-9464","authenticated-orcid":false,"given":"Luigi","family":"Lucini","sequence":"additional","affiliation":[{"name":"Department for Sustainable Food Process Universit\u00e0 Cattolica del Sacro Cuore  Piacenza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3165-4579","authenticated-orcid":false,"given":"Paolo","family":"Ajmone\u2010Marsan","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Food and Nutrition\u2013DIANA and Proteomics and Nutrigenomics Research Center\u2010PRONUTRIGEN Universit\u00e0 Cattolica del Sacro Cuore  Piacenza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5819-0861","authenticated-orcid":false,"given":"Maur\u00edcio F.","family":"van Tilburg","sequence":"additional","affiliation":[{"name":"Department of Animal Science \u2013 Federal Rural University of the Semi\u2010Arid Mossor\u00f3 Rio Grande do Norte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8271-5733","authenticated-orcid":false,"given":"Arlindo A.","family":"Moura","sequence":"additional","affiliation":[{"name":"Department of Animal Science Federal University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2560251"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767050903181500"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod47.2.297"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.07.009"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1200001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deq024"},{"key":"e_1_2_8_8_1","first-page":"66","article-title":"Addition of hypotaurine can reactivate immotile golden hamster spermatozoa","volume":"11","author":"Boatman D. 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