{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T11:44:16Z","timestamp":1778931856491,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T00:00:00Z","timestamp":1597881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cell cycle of mouse embryo could be delayed by nicotinamide (NAM). Histone H3 lysine 56 (H3K56ac) acetylation plays an important role in mammalian genomic stability and the function of this modification in mouse embryos is not known. Hence, we designed to study the effects of NAM\u2010induced oxidative stress on the developmental ability of mouse embryos, on the acetylation of H3K56ac and the possible functions of this modification related to mouse embryo development. Treatment with NAM (10, 20, or 40\u2009mmol\/L for 24 or 48\u2009hr) during in vitro culture significantly decreased developmental rate of blastocyst (24\u2009hr: 90.2 vs. 81.2, 43.2, and 18.2, with <jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01, respectively; 48\u2009hr: 89.3 vs. 53.2%, 12.1%, and 0% with <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05, respectively). NAM treatment (20\u2009mmol\/L) for 6 and 31\u2009hr resulted in increased intracellular reactive oxygen species levels in two\u2010cell embryos, and apoptotic cell numbers in blastocysts. Resveratrol (RSV) and I\u2010CBP112 rescued the 20\u2009mmol\/L NAM\u2010induced embryo developmental defects. RSV and I\u2010CBP112 increased the level of Sirt1 and decreased the level of H3K56ac induced by NAM in two\u2010cell embryos (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). These data suggest that NAM treatment decreases the expression of Sirt1, which induces high levels of H3K56 acetylation that may be involved in oxidative stress\u2010induced mouse embryo defects, which can be rescued by RSV and I\u2010CBP112.<\/jats:p>","DOI":"10.1002\/mrd.23405","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T04:59:11Z","timestamp":1598590751000},"page":"1009-1017","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Nicotinamide\u2010induced mouse embryo developmental defect rescued by resveratrol and I\u2010CBP112"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5817-4247","authenticated-orcid":false,"given":"Yu\u2010Guo","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Theriogenology, College of Veterinary Medicine\/Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety, The Ministry of Education of China Yangzhou University  Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses\/Jiangsu Key Laboratory of Zoonosis\/Jiangsu Key Laboratory of Animal genetic Breeding and Molecular Design, College of Animal Science and Technology Yangzhou University  Yangzhou Jiangsu China"},{"name":"Department of Stem cell and Regenerative Biotechnology Konkuk University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, College of Veterinary Medicine\/Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety, The Ministry of Education of China Yangzhou University  Yangzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6448-1160","authenticated-orcid":false,"given":"Ayman","family":"Mesalam","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine Zagazig University  Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, College of Veterinary Medicine\/Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety, The Ministry of Education of China Yangzhou University  Yangzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Jung","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Stem cell and Regenerative Biotechnology Konkuk University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abu Musa 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