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In the present study, we explored the effects of short\u2010term NAM\u2010treatment (3 and 5\u2009h) during in vitro fertilization (IVF) on the development of bovine embryos. Treatment with 10\u2009mM NAM for 3\u2009h\u00a0significantly increased the blastocyst formation but extending the treatment to 5\u2009h\u00a0did not enhance the benefits any further. Immunofluorescence analysis demonstrated that treatment with 10\u2009mM NAM for 3\u2009h\u00a0decreased the expression of intracellular ROS, 8\u2010oxo\u20107,8\u2010dihydroguanine, caspase\u20103, and increased the expression of Sirt1, and incorporation of bromodeoxyuridine in one\u2010cell stage embryos. Similarly, the level of H3K56ac significantly increased in the NAM\u2010treated (3 and 5\u2009h) one\u2010cell stage embryos. Contrastingly, the treatment with 10\u2009mM NAM for 5\u2009h\u00a0increased the caspase\u20109 level in blastocysts. Collectively, these findings suggest that NAM possesses antioxidant activity and supplementation of IVF medium with 10\u2009mM NAM for 3\u2009h\u00a0improves\u00a0the in vitro developmental competence of bovine embryos.<\/jats:p>","DOI":"10.1002\/mrd.23417","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T10:47:09Z","timestamp":1599216429000},"page":"1070-1081","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of nicotinamide supplementation in in vitro fertilization medium on bovine embryo development"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5817-4247","authenticated-orcid":false,"given":"Yu\u2010Guo","family":"Yuan","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine\/Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6448-1160","authenticated-orcid":false,"given":"Ayman","family":"Mesalam","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok\u2010Hwan","family":"Song","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21 Plus) Gyeongsang National University Jinju Gyeongnam Republic of Korea"},{"name":"Institute of Agriculture and Life Science Gyeongsang National University Jinju Gyeongnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeong\u2010Lim","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21 Plus) Gyeongsang National University Jinju Gyeongnam Republic of Korea"},{"name":"Institute of Agriculture and Life Science Gyeongsang National University Jinju Gyeongnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2581-5567","authenticated-orcid":false,"given":"Lianguang","family":"Xu","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21 Plus) Gyeongsang National University Jinju Gyeongnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myeong\u2010Don","family":"Joo","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21 Plus) Gyeongsang National University Jinju Gyeongnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2333-134X","authenticated-orcid":false,"given":"Il\u2010Keun","family":"Kong","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21 Plus) Gyeongsang National University Jinju Gyeongnam Republic of Korea"},{"name":"The King Kong Corp. 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