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We investigate here the possible roles, immunolocalizations, and synthesis of the Wnt3, Wnt7a, and beta\u2010catenin proteins in the rat endometrium during the estrous cycle and early postimplantation period. Wnt3 and Wnt7a had a similar localization and dynamic expression relative to the endometrial stages. Wnt7a immunostaining was not limited only to the luminal epithelial cells, but also to strong stainings in the stromal and endothelial cells. Wnt3, Wnt7a, and beta\u2010catenin were highly synthesized and colocalized at the trophoblast\u2010decidual interface; and were more obvious in the primary decidual zone, the GTCs, and the ectoplacental cone. Beta\u2010catenin was strongly localized at the borders of the mature decidual cells; however, Wnt3 and Wnt7a immunolocalizations were decreased in those cells. As such, the immunolocalization of Wnt3, Wnt7a, and beta\u2010catenin shifted with decidualization and placentation. The expression level of Wnt3, Wnt7a, and beta\u2010catenin messenger RNAs increased in early pregnancy, and especially between Days 8.5 and 9.5. The dramatic changes in the expression of Wnt3, Wnt7a, and beta\u2010catenin observed during the early days of pregnancy and the estrous cycle may indicate their roles in decidualization, stromal cell proliferation, and trophoblast invasion.<\/jats:p>","DOI":"10.1002\/mrd.23423","type":"journal-article","created":{"date-parts":[[2020,9,19]],"date-time":"2020-09-19T10:51:08Z","timestamp":1600512668000},"page":"1159-1172","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Wingless ligands and beta\u2010catenin expression in the rat endometrium: The role of Wnt3 and Wnt7a\/beta\u2010catenin pathway at the embryo\u2013uterine interface"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9428-5259","authenticated-orcid":false,"given":"Celal","family":"Kalo\u011flu","sequence":"first","affiliation":[{"name":"Assisted Reproduction Technology (ART) Center, Faculty of Medicine Sivas\u2010Cumhuriyet University  Sivas Turkey"},{"name":"Department of Histology and Embryology, Faculty of Medicine Sivas\u2010Cumhuriyet University  Sivas Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2851-8808","authenticated-orcid":false,"given":"H\u00fcseyin E.","family":"Bulut","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Sivas\u2010Cumhuriyet University  Sivas Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2474-5336","authenticated-orcid":false,"given":"Rasim","family":"Hamuto\u011flu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Sivas\u2010Cumhuriyet University  Sivas Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0699-1354","authenticated-orcid":false,"given":"Ertan M.","family":"Korkmaz","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science Sivas\u2010Cumhuriyet University  Sivas Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1328-560X","authenticated-orcid":false,"given":"Ozan","family":"\u00d6nder","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Sivas\u2010Cumhuriyet University  Sivas Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3616-1183","authenticated-orcid":false,"given":"Tu\u011fba","family":"Da\u011fdeviren","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Sivas\u2010Cumhuriyet University  Sivas Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6328-626X","authenticated-orcid":false,"given":"Merve N.","family":"Aydemir","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science Sivas\u2010Cumhuriyet University  Sivas Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.13.3797"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402660610"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s10930-020-09888-y","article-title":"Assessment of protein profiles of RNAlater stored and fresh PBMC cells using different protein extraction buffers","volume":"39","author":"Alyethodi R. 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