{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:26:45Z","timestamp":1761920805319,"version":"3.37.3"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T00:00:00Z","timestamp":1601942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HD083311"],"award-info":[{"award-number":["R01HD083311"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In eukaryotic cells, RNA polymerase (Pol) I and Pol III are dedicated to the synthesis of ribosomal RNA precursors and a variety of small RNAs, respectively. Although RNA Pol I and Pol III complexes are crucial for the regulation of cell growth and cell cycle in all cell types, many of the components of the Pol I and Pol III complexes have not been functionally characterized in mammals. Here, we provide the first in vivo functional characterization of POLR1D, a subunit shared by RNA Pol I and Pol III, during early mammalian embryo development. Our results show that <jats:italic>Polr1d<\/jats:italic> mutant embryos cannot be recovered at E7.5 early post\u2010gastrulation stage, suggesting failed implantation. Although <jats:italic>Polr1d<\/jats:italic> mutants can be recovered at E3.5, they exhibit delayed\/stalled development with morula morphology rather than differentiation into blastocysts. Even with extended time in culture, mutant embryos fail to form blastocysts and eventually die. Analysis of E3.0 embryos revealed severe DNA damage in <jats:italic>Polr1d<\/jats:italic> mutants. Additionally, lineage assessment reveals that trophectoderm specification is compromised in the absence of <jats:italic>Polr1d<\/jats:italic>. In summary, these findings demonstrate the essential role of POLR1D during early mammalian embryogenesis and highlight cell\u2010lethal phenotype without <jats:italic>Polr1d<\/jats:italic> function.<\/jats:p>","DOI":"10.1002\/mrd.23427","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T19:49:35Z","timestamp":1602013775000},"page":"1152-1158","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Loss of POLR1D results in embryonic lethality prior to blastocyst formation in mice"],"prefix":"10.1002","volume":"87","author":[{"given":"Xiaosu","family":"Miao","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tieqi","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morgane","family":"Golan","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesse","family":"Mager","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7281-3471","authenticated-orcid":false,"given":"Wei","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts  Amherst Massachusetts USA"},{"name":"Animal Models Core Facility, Institute for Applied Life Sciences (IALS) University of Massachusetts  Amherst Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)59545-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-019-0105-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(02)00127-6"},{"issue":"4","key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"C419","DOI":"10.1152\/ajpcell.1988.255.4.C419","article-title":"Mammalian blastocyst: Transport functions in a developing epithelium","volume":"255","author":"Biggers J. 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