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Exogenous plasminogen activator streptokinase (SK) added to the IVM medium revealed similar values of cumulus expansion and oocyte nuclear maturation compared to controls (standard IVM medium). However, a decrease in both determinations was observed in COCs matured with the supplementation of \u025b\u2010aminocaproic acid (\u025b\u2010ACA), a specific plasmin inhibitor. After in vitro fertilization, no differences were observed in either cleavage or blastocyst rates between SK and control groups; however, \u03b5\u2010ACA treatment caused a decrease in both developmental rates. Zona pellucida (ZP) digestion time decreased in the SK group while it increased in the \u03b5\u2010ACA group. Raman microspectroscopy revealed an increase in the intensity of the band corresponding to the glycerol group of sialic acid in the ZP of oocytes matured with SK, whereas ZP spectra of oocytes treated with \u025b\u2010ACA presented similarities with immature oocytes. The results indicate that although treatment with SK did not alter oocyte developmental competence, it induced modifications in the ZP of oocytes that could modify the folding of glycoproteins. Plasmin inhibition impairs oocyte maturation and has an impact on embryo development, thus evidencing the importance of this protease during IVM.<\/jats:p>","DOI":"10.1002\/mrd.23441","type":"journal-article","created":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T07:05:33Z","timestamp":1606460733000},"page":"67-79","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exogenous activation and inhibition of plasminogen\/plasmin activity during in vitro maturation of bovine cumulus\u2010oocyte complexes: A biological and spectroscopic approach"],"prefix":"10.1002","volume":"88","author":[{"given":"Gabriela","family":"Rizo","sequence":"first","affiliation":[{"name":"Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO) \u00c1rea Biolog\u00eda Experimental, CONICET\u2010Universidad Nacional de Tucuman  Tucum\u00e1n Argentina"},{"name":"Instituto de Biolog\u00eda \u2018Dr. Francisco D. Barbieri\u2019, Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia Universidad Nacional de Tucum\u00e1n  Tucum\u00e1n Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5258-1693","authenticated-orcid":false,"given":"Antonio Daniel","family":"Barrera","sequence":"additional","affiliation":[{"name":"Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO) \u00c1rea Biolog\u00eda Experimental, CONICET\u2010Universidad Nacional de Tucuman  Tucum\u00e1n Argentina"},{"name":"Instituto de Biolog\u00eda \u2018Dr. Francisco D. Barbieri\u2019, Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia Universidad Nacional de Tucum\u00e1n  Tucum\u00e1n Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Emanuel","family":"Jimenez","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica del Noroeste Argentino (INQUINOA) CONICET\u2010Universidad Nacional de Tucuman  Tucum\u00e1n Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6472-329X","authenticated-orcid":false,"given":"Elina Vanesa","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO) \u00c1rea Biolog\u00eda Experimental, CONICET\u2010Universidad Nacional de Tucuman  Tucum\u00e1n Argentina"},{"name":"Instituto de Biolog\u00eda \u2018Dr. Francisco D. Barbieri\u2019, Facultad de Bioqu\u00edmica, Qu\u00edmica y Farmacia Universidad Nacional de Tucum\u00e1n  Tucum\u00e1n Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9616-0786","authenticated-orcid":false,"given":"Daniela C.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto Superior de Investigaciones Biol\u00f3gicas (INSIBIO) \u00c1rea Biolog\u00eda Experimental, CONICET\u2010Universidad Nacional de Tucuman  Tucum\u00e1n Argentina"},{"name":"Instituto de Biolog\u00eda \u2018Dr. Francisco D. 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