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This condition, characterized by an imbalance in the levels of oxidizing and antioxidant species in the seminal fluid, has a harmful impact on sperm functions and DNA integrity. The present study aimed to evaluate the anti\u2010genotoxic action of ellagic acid, a polyphenolic molecule of natural origin having a powerful antigenotoxic, anti\u2010inflammatory and antiproliferative role. An OS condition was induced in vitro by incubating normozoospermic human semen samples in benzene for 45, 60 and 90 min. DNA integrity was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling assay, RAPD\u2010PCR was performed to calculate the genome template stability, while the percentage of intracellular reactive oxygen species (ROS) was assessed by the 2\u02b9, 7\u02b9\u2010dichlorofluorescein assay. Our results showed that ellagic acid has a consistent protective effect on DNA integrity, as well as on sperm vitality and motility, by counteracting generation of intracellular ROS. The results of this study suggest ellagic acid as a suitable molecule to protect sperm DNA from oxidative stress, with a potentially significant translational impact on the management of the male infertility.<\/jats:p>","DOI":"10.1002\/mrd.23455","type":"journal-article","created":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T03:13:41Z","timestamp":1612149221000},"page":"167-174","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["In vitro ameliorative effects of ellagic acid on vitality, motility and DNA quality in human spermatozoa"],"prefix":"10.1002","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9636-5842","authenticated-orcid":false,"given":"Concetta","family":"Iovine","sequence":"first","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9228-9398","authenticated-orcid":false,"given":"Filomena","family":"Mottola","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0445-4136","authenticated-orcid":false,"given":"Marianna","family":"Santonastaso","sequence":"additional","affiliation":[{"name":"Department of Woman, Child and General and Special Surgery University of Campania \u201cLuigi Vanvitelli\u201d  Napoli Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5926-6407","authenticated-orcid":false,"given":"Renata","family":"Finelli","sequence":"additional","affiliation":[{"name":"American Center of Reproductive Medicine, Andrology Center Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0585-1026","authenticated-orcid":false,"given":"Ashok","family":"Agarwal","sequence":"additional","affiliation":[{"name":"American Center of Reproductive Medicine, Andrology Center Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6250-4798","authenticated-orcid":false,"given":"Lucia","family":"Rocco","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.190055"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.2014.32.1.1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)61730-0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2006.06.001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4196\/kjpp.2016.20.3.269"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4196\/kjpp.2016.20.3.269"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2011.01.006"},{"issue":"9","key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.acuro.2014.02.022","article-title":"Fragmentaci\u00f3n del ADN esperm\u00e1tico empleando el m\u00e9todo de TUNEL","volume":"38","author":"Chenlo P. 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