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However, in the conventional method, ciPSCs are maintained using chemically\u2010undefined media containing unknown animal components under on\u2010murine embryonic fibroblast feeder conditions, which were reported to modify cell surface of iPSCs and increases the risk of immune rejection when the cells are transplanted into patients. Moreover, in the conventional method, ciPSCs are mechanically passaged, which requires much time and effort. Therefore, the large\u2010scale expansion of ciPSCs is difficult, which should be resolved for using ciPSCs in clinical application and research. Here, it was shown that StemFit\u00ae AK02N and iMatrix\u2010511 could maintain the pluripotency of ciPSCs using conventional culture method. Furthermore, it was demonstrated that the feeder\u2010free and chemically\u2010defined ciPSC culture systems using StemFit\u00ae AK02N and iMatrix\u2010511 could stably maintain and allow the easy expansion of ciPSCs generated using N2B27 and StemFit\u00ae AK02N, without causing karyotype abnormalities. ciPSCs expressed several pluripotency markers and formed teratomas, including cells derived from three germ layers.<\/jats:p>","DOI":"10.1002\/mrd.23478","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T17:56:06Z","timestamp":1621446966000},"page":"395-404","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Canine induced pluripotent stem cell maintenance under feeder\u2010free and chemically\u2010defined conditions"],"prefix":"10.1002","volume":"88","author":[{"given":"Kazuto","family":"Kimura","sequence":"first","affiliation":[{"name":"Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences Osaka Prefecture University  Izumisano Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaya","family":"Tsukamoto","sequence":"additional","affiliation":[{"name":"Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences Osaka Prefecture University  Izumisano Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takumi","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences Osaka Prefecture University  Izumisano Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miyuu","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Integrated Structural Biosciences, Graduate School of Life and Environmental Sciences Osaka Prefecture University  Izumisano Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuru","family":"Kuwamura","sequence":"additional","affiliation":[{"name":"Department of Integrated Structural Biosciences, Graduate School of Life and Environmental Sciences Osaka Prefecture University  Izumisano Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manami","family":"Ohtaka","sequence":"additional","affiliation":[{"name":"TOKIWA\u2010Bio, Inc.  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Laboratory of Gene Regulation, Faculty of Medicine University of Tsukuba  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahito","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"TOKIWA\u2010Bio, Inc.  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