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To investigate this, we established a hypercortisolism mouse model by supplementing the drinking water with corticosterone for four weeks. In the hypercortisolism mice, the serum corticosterone was much higher than in the control, and serum testosterone was significantly decreased. Moreover, corticosterone treatment induced decrease of sperm counts and increase of teratozoospermia. Increased numbers of multinucleated giant cells and apoptotic germ cells as well as downregulated meiotic markers suggested that corticosterone induced impaired spermatogenesis. Further, upregulation of macrophage\u2010specific marker antigen F4\/80 as well as inflammation\u2010related genes suggested that corticosterone induced inflammation in the testis. Lactate content was found to be decreased in the testis and Sertoli cells after corticosterone treatment, and lactate metabolism\u2010related genes were downregulated. In vitro phagocytosis assays showed that the phagocytic activity in corticosterone\u2010treated Sertoli cells was downregulated and accompanied by decreased mitochondrial membrane potential, while pyruvate dehydrogenase kinase\u20104 inhibitor supplementation restored this process. Taken together, our results demonstrated that dysfunctional phagocytosis capacity and lactate metabolism in Sertoli cells participates in corticosterone\u2010induced impairment of spermatogenesis.<\/jats:p>","DOI":"10.1002\/mrd.23515","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T05:09:26Z","timestamp":1621919366000},"page":"405-415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Dysfunction in Sertoli cells participates in glucocorticoid\u2010induced impairment of spermatogenesis"],"prefix":"10.1002","volume":"88","author":[{"given":"Li","family":"Ren","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Physiology &amp; Biochemistry, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine Nanjing Agricultural University  Nanjing 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