{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T16:08:16Z","timestamp":1784045296861,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T00:00:00Z","timestamp":1627344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002951","name":"Dokkyo Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002951","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002951","name":"Dokkyo Medical University","doi-asserted-by":"publisher","award":["21K09378"],"award-info":[{"award-number":["21K09378"]}],"id":[{"id":"10.13039\/501100002951","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mammalian sperm have to undergo capacitation to fertilize the egg. At the molecular level, capacitation involves cAMP synthesis, protein kinase A activation, and downstream increase in tyrosine phosphorylation. In addition, during capacitation, mammalian sperm actively generate reactive oxygen species (ROS). It has been proposed that ROS modulate phosphorylation pathways; however, the crosstalk between these signaling processes is not well\u2010understood. In the present study, we used loss\u2010 and gain\u2010of\u2010function approaches to evaluate the interconnection between ROS and phosphorylation. We showed that BSA and HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>, but not Ca<jats:sup>2+<\/jats:sup>, in the capacitation media are required for ROS production. The synergic effect of these compounds was neither mediated by HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> stimulation of cAMP synthesis nor by BSA\u2010induced cholesterol efflux. The capacitation\u2010induced ROS generation was blocked in the presence of superoxide dismutase (SOD), catalase, and apocynin. However, none of these compounds affected cAMP\u2010dependent or tyrosine phosphorylation. On the other hand, the addition of NADPH to the media induced ROS generation in sperm incubated in the absence of BSA and HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> without upregulating cAMP\u2010dependent or tyrosine phosphorylation signaling. Most interestingly, catalase, but not SOD, blocked in vitro fertilization suggesting a role for H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> in this process.<\/jats:p>","DOI":"10.1002\/mrd.23524","type":"journal-article","created":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T05:25:29Z","timestamp":1627449929000},"page":"544-557","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Activation of cAMP\u2010dependent phosphorylation pathways is independent of ROS production during mouse sperm capacitation"],"prefix":"10.1002","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0151-7875","authenticated-orcid":false,"given":"Gen L.","family":"Takei","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Massachusetts Amherst Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darya 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