{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:51:02Z","timestamp":1786978262140,"version":"build-2736575974"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The present work aimed to explore let\u20107b's molecular mechanisms that regulate the functions of placental trophoblasts and to examine placental let\u20107b expression in human pre\u2010eclampsia (PE). Human trophoblast HTR\u20108\/SVneo cells underwent transduction with control and let\u20107b overexpressing lentiviruses, respectively. Cell proliferation assessment utilized cell counting kit\u20108 (CCK\u20108) and 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine (EdU) assays. Apoptosis, autophagy, inflammation, epithelial\u2010to\u2010mesenchymal transition (EMT), and ERK1\/2 signaling\u2010associated proteins were assessed by immunoblot. Placental tissue samples were collected from women with normal pregnancy (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200920) and PE patients (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200914). Let\u20107b overexpression in HTR\u20108\/SVneo cells remarkably induced cell proliferation and invasion, suppressed apoptosis and autophagy, and resulted in decreased tumor necrosis factor\u03b1 (TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    ) expression and increased interleukin 6 (IL\u20106) expression in trophoblasts. Notably, the beneficial effects of let\u20107b overexpression, including cell invasion and EMT, were largely reversed by treatment with U0126, an indirect ERK1\/2 signaling inhibitor, in these cells. TGF\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    receptor type\u20101 (TGFBR1) overexpression weakened let\u20107b's functions in ERK pathway activation and invasion in trophoblasts. Placental tissue specimens from PE cases demonstrated significantly lower let\u20107b expression compared with normal controls. Overexpression of let\u20107b exerts beneficial effects on the functions of human placental trophoblasts via ERK1\/2 signaling, and placental let\u20107b is downregulated in human PE. These findings suggest let\u20107b is a promising biomarker for the prospective diagnosis and targeted therapy of PE.\n                  <\/jats:p>","DOI":"10.1002\/mrd.23535","type":"journal-article","created":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T06:07:21Z","timestamp":1632290841000},"page":"39-53","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Overexpression of let\u20107b exerts beneficial effects on the functions of human placental trophoblasts by activating the ERK1\/2 signaling pathway"],"prefix":"10.1002","volume":"89","author":[{"given":"Yanyan","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Obstetrics The First Affiliated Hospital of China Medical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency Shen Yang Red Cross Hospital  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics The First Affiliated Hospital of China Medical University  Shenyang 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