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However, intercellular messengers regulating signaling events, like proliferation in the OSE, have not been completely described. Purines have emerged as novel intercellular messengers in the ovary, in which expression of purinergic receptors has been reported in different cell types. In the present work, we described the functional expression of P2Y2 receptor (P2Y2R), a purinergic receptor widely associated with cell proliferation, in the OSE. The expression of P2Y2R by immunofluorescence and RT\u2010PCR, and its functionality by Ca<jats:sup>2+<\/jats:sup>recording was demonstrated in primary cultured OSE. Functional expression of P2Y2R was also exhibited in situ, by recording of intracellular Ca<jats:sup>2+<\/jats:sup>release and detection of ERK phosphorylation after injection of a selective agonist into the ovarian bursa. Furthermore, P2Y2R activation with UTP\u03b3S, in situ, induced cell proliferation at 24\u2009h, whereas continuous stimulation of P2Y2R during a complete estrous cycle significantly modified the size distribution of the follicular population. This is the first evidence of the functional expression of purinergic P2Y2R in the OSE and opens new perspectives on the roles played by purines in ovarian physiology.<\/jats:p>","DOI":"10.1002\/mrd.23545","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T11:58:41Z","timestamp":1635163121000},"page":"758-770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional expression of P2Y2 receptors in mouse ovarian surface epithelium (OSE)"],"prefix":"10.1002","volume":"88","author":[{"given":"Ana Patricia","family":"Ju\u00e1rez\u2010Mercado","sequence":"first","affiliation":[{"name":"Departamento de Neurobiolog\u00eda Celular y Molecular, Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico Quer\u00e9taro M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebeca","family":"Ch\u00e1vez\u2010Genaro","sequence":"additional","affiliation":[{"name":"Departamento de Histolog\u00eda y Embriolog\u00eda, Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiana","family":"Fiordelisio","sequence":"additional","affiliation":[{"name":"Laboratorio de Neuroendocrinolog\u00eda Comparada, Departamento de Ecolog\u00eda y Recursos Naturales, Biolog\u00eda, Facultad de Ciencias, Universidad Nacional Aut\u00f3noma de M\u00e9xico Ciudad Universitaria CDMX M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Gonz\u00e1lez\u2010Gallardo","sequence":"additional","affiliation":[{"name":"Departamento de Neurobiolog\u00eda Celular y Molecular, Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico Quer\u00e9taro M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauricio","family":"D\u00edaz\u2010Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Departamento de Neurobiolog\u00eda Celular y Molecular, Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico Quer\u00e9taro M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8248-332X","authenticated-orcid":false,"given":"Francisco G.","family":"V\u00e1zquez\u2010Cuevas","sequence":"additional","affiliation":[{"name":"Departamento de Neurobiolog\u00eda Celular y Molecular, Instituto de Neurobiolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico Quer\u00e9taro M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,25]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_9_2_1","DOI":"10.1016\/j.fertnstert.2009.01.127"},{"doi-asserted-by":"publisher","key":"e_1_2_9_3_1","DOI":"10.1095\/biolreprod.102.003889"},{"doi-asserted-by":"publisher","key":"e_1_2_9_4_1","DOI":"10.1097\/PGP.0b013e3182800ad5"},{"issue":"2","key":"e_1_2_9_5_1","first-page":"255","article-title":"Ovarian surface epithelium: Biology, endocrinology, and pathology","volume":"22","author":"Auersperg N.","year":"2001","journal-title":"Endocrine Reviews"},{"doi-asserted-by":"publisher","key":"e_1_2_9_6_1","DOI":"10.1093\/biolre\/ioy250"},{"issue":"8","key":"e_1_2_9_7_1","first-page":"11508","article-title":"P2Y6 purinergic receptor regulates steroid synthesis and proliferation of ovarian luteal cells","volume":"10","author":"Bao R.","year":"2017","journal-title":"International Journal of Clinical and Experimental Medicine"},{"doi-asserted-by":"publisher","key":"e_1_2_9_8_1","DOI":"10.1016\/0304-3835(94)90348-4"},{"doi-asserted-by":"publisher","key":"e_1_2_9_9_1","DOI":"10.1530\/jrf.0.0190223"},{"doi-asserted-by":"publisher","key":"e_1_2_9_10_1","DOI":"10.1210\/en.2005-1629"},{"doi-asserted-by":"publisher","key":"e_1_2_9_11_1","DOI":"10.1038\/cddis.2009.11"},{"issue":"1700","key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1098\/rstb.2015.0422","article-title":"Short\u2010 and long\u2010term (trophic) purinergic signalling","volume":"371","author":"Burnstock G.","year":"2016","journal-title":"Philosophical Transactions of the Royal Society of London, Series B: Biological Sciences"},{"doi-asserted-by":"publisher","key":"e_1_2_9_13_1","DOI":"10.1002\/mrd.23106"},{"doi-asserted-by":"publisher","key":"e_1_2_9_14_1","DOI":"10.1016\/j.bbamcr.2006.11.010"},{"doi-asserted-by":"publisher","key":"e_1_2_9_15_1","DOI":"10.1210\/jcem.87.5.8506"},{"doi-asserted-by":"publisher","key":"e_1_2_9_16_1","DOI":"10.1095\/biolreprod.102.006551"},{"key":"e_1_2_9_17_1","first-page":"1","article-title":"In vivo imaging and therapeutic treatments in an orthotopic mouse model of ovarian cancer","volume":"42","author":"Cordero A. 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