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However, the expression profiles of some members of this protein family and their effect on sperm quality remain unclear. In this study, we found that GST <jats:italic>kappa<\/jats:italic> 1 (GSTK1) encoded protein is abundant in the testes and capacitated sperm acrosome. Western blot analysis revealed that the decreased abundance of GSTK1 was observed in low motile spermatozoa; moreover, GSTK1 expression decreased in sperm stored at 17\u00b0C under a long preservation time. In vitro analyses revealed that GSTK1 had no significant effect on sperm motility, capacitation, or acrosome reaction. Notably, after capacitated sperm were incubated with 4 and 8\u2009\u03bcg\/ml anti\u2010GSTK1 antibodies, the fertilization rate significantly decreased in vitro fertilization assay. The current study demonstrates that GSTK1 is correlated with sperm quality and is a promising marker for the assessment of sperm quality and provides a basis for understanding the potential molecular mechanism for targeting pathogenic factors in male infertility.<\/jats:p>","DOI":"10.1002\/mrd.23551","type":"journal-article","created":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T06:09:09Z","timestamp":1639116549000},"page":"104-112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Glutathione S\u2010transferase kappa 1 is positively related with sperm quality of porcine sperm"],"prefix":"10.1002","volume":"89","author":[{"given":"Fanwen","family":"Zeng","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro\u2010animal Genomics and Molecular Breeding College of Animal Science of South China Agricultural University 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