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To date, the profiling of FF proteomes during follicle selection, development, and ovulation has not been comprehensively investigated. Therefore, a shotgun proteomics approach and bioinformatics analyses were used to profile the proteomes of equine FF harvested in vivo from follicles at the following development stages: predeviation (18\u201320\u2009mm), deviation (22\u201325\u2009mm), postdeviation (26\u201329\u2009mm), preovulatory (30\u201335\u2009mm), and impending ovulation. A total of 294 proteins were detected in FF (FDR\u2009&lt;1%), corresponding to 65 common proteins and 124, 142, 167, 132, and 142 proteins in the predeviation, deviation, postdeviation, preovulatory, and impending ovulation groups, respectively. The higher expression of properdin and several other proteins belonging to the complement system during the deviation time and ovulation suggested their contribution in the selection of the future dominant follicle and ovulation. Apolipoprotein A\u20101 and antithrombin\u2010III appeared to be important throughout folliculogenesis. The \u201ccomplement and coagulation cascades\u201d was the major KEGG pathway across all stages of follicle development. The significant expression of several proteins belonging to the serine\u2010type endopeptidase indicated their likely contribution to follicle and oocyte development. Our data provide an extensive description and functional analyses of the equine FF proteome during follicle selection, development, and ovulation. This information will help improve understanding of the ovarian function and ovulatory dysfunctions and might serve as a reference for future biomarker discovery for oocyte quality assessment.<\/jats:p>","DOI":"10.1002\/mrd.23622","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T06:20:10Z","timestamp":1656397210000},"page":"298-311","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Follicular\u2010fluid proteomics during equine follicle development"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3448-0578","authenticated-orcid":false,"given":"Ghassan M.","family":"Ishak","sequence":"first","affiliation":[{"name":"Department of Surgery and Obstetrics, College of Veterinary Medicine University of Baghdad  Baghdad Iraq"},{"name":"Animal Science, School of Agricultural Sciences Southern Illinois University  Carbondale Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1376-5059","authenticated-orcid":false,"given":"Jean M.","family":"Feugang","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences Mississippi State University  Mississippi State Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Pechanova","sequence":"additional","affiliation":[{"name":"Institute for Genomics, Biocomputing &amp; Biotechnology Mississippi State University  Mississippi State Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tibor","family":"Pechan","sequence":"additional","affiliation":[{"name":"Institute for Genomics, Biocomputing &amp; Biotechnology Mississippi State University  Mississippi State Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Peterson","sequence":"additional","affiliation":[{"name":"Institute for Genomics, Biocomputing &amp; Biotechnology Mississippi State University  Mississippi State Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott T.","family":"Willard","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences Mississippi State University  Mississippi State Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter L.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences Mississippi State University  Mississippi State Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8855-6980","authenticated-orcid":false,"given":"Eduardo L.","family":"Gastal","sequence":"additional","affiliation":[{"name":"Animal Science, School of Agricultural Sciences Southern Illinois University  Carbondale Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.04.010"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.9.1.10266"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.05.017"},{"key":"e_1_2_8_5_1","first-page":"20","article-title":"Human antral folliculogenesis: What we have learned from the bovine and equine models","volume":"6","author":"Baerwald A. 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