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Our previous bioinformatics analysis predicted that RBP4 is a target of the microRNA miRNA\u201024\u20103p. In this study, our database analysis also indicated that RBP4 is a miR\u201024\u20103p target. Compared with that of the normal placenta, the expression level of RBP4 in human PE placenta was significantly reduced, and miR\u201024\u20103p was highly expressed. In HTR\u20108\/SVneo cells, transfection of exogenous miR\u201024\u20103p reduced RBP4 expression. A dual\u2010luciferase reporter assay validated RBP4 as a direct target of miR\u201024\u20103p, indicating that it directly binds to the 3\u2032\u2010untranslated region of RBP4. This binding was reversed by a mutation in the microRNA\u2010binding site. Transwell invasion experiments and CCK8 assay showed that inhibitory effect of miR\u201024\u20103p reduced RBP4 mediated HTR\u20108\/SVneo cell invasion and proliferation. These data provide a new overarching perspective on the physiological role played by miR\u201024\u20103p in regulating RBP4 during trophoblast dysfunction and PE development.<\/jats:p>","DOI":"10.1002\/mrd.23633","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T09:45:45Z","timestamp":1657619145000},"page":"423-430","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["miR\u201024\u20103p regulation of retinol binding protein 4 in trophoblast biofunction and preeclampsia"],"prefix":"10.1002","volume":"89","author":[{"given":"Zhan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Beijing Chaoyang Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Ru","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Beijing Chaoyang Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Beijing Chaoyang Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangming","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Beijing Chaoyang Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/01443615.2020.1820465"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.369132"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.25100\/cm.v46i4.1887"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.07.021"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/09674845.2020.1769331"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641955.2016.1239736"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S273146"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.931714"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/13816810.2020.1755985"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-109099"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-017-4378-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2021.09.007"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.785219"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-16-0969"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.25011\/cim.v38i5.25684"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20200023"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753495X15585257"},{"issue":"4","key":"e_1_2_9_19_1","first-page":"2137","article-title":"Correlation of miRNA and VEGF expression with the outcome of early\u2010onset severe preeclampsia in patients receiving expectant treatment","volume":"11","author":"Han L.","year":"2018","journal-title":"International journal of clinical and experimental pathology"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203721666200210102751"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2021.09.004"},{"key":"e_1_2_9_22_1","first-page":"3","article-title":"Association of circulating miRNA expression with preeclampsia, its onset, and severity","volume":"11","author":"Kolkova Z.","year":"2021","journal-title":"Diagnostics (Basel)"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767058.2017.1296426"},{"issue":"3","key":"e_1_2_9_24_1","first-page":"2873","article-title":"RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K\/AKT signaling pathway","volume":"18","author":"Li H.","year":"2018","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01370.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.K10E-288"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641955.2021.1975737"},{"key":"e_1_2_9_28_1","doi-asserted-by":"crossref","unstructured":"Medjedovic E. 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