{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:23:29Z","timestamp":1790619809564,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Fetal growth restriction (FGR) significantly contributes to neonatal and perinatal morbidity and mortality. Currently, there are no effective treatment options for FGR during pregnancy. We have developed a nanoparticle gene therapy targeting the placenta to increase expression of human\n                    <jats:italic>insulin\u2010like growth factor 1<\/jats:italic>\n                    (\n                    <jats:italic>hIGF1<\/jats:italic>\n                    ) to correct fetal growth trajectories. Using the maternal nutrient restriction guinea pig model of FGR, an ultrasound\u2010guided, intraplacental injection of nonviral, polymer\u2010based\n                    <jats:italic>hIGF1<\/jats:italic>\n                    nanoparticle containing plasmid with the\n                    <jats:italic>hIGF1<\/jats:italic>\n                    gene and placenta\u2010specific\n                    <jats:italic>Cyp19a1<\/jats:italic>\n                    promotor was administered at mid\u2010pregnancy. Sustained\n                    <jats:italic>hIGF1<\/jats:italic>\n                    expression was confirmed in the placenta 5 days after treatment. Whilst increased\n                    <jats:italic>hIGF1<\/jats:italic>\n                    did not change fetal weight, circulating fetal glucose concentration were 33%\u201367% higher. This was associated with increased expression of glucose and amino acid transporters in the placenta. Additionally,\n                    <jats:italic>hIGF1<\/jats:italic>\n                    nanoparticle treatment increased the fetal capillary volume density in the placenta, and reduced interhaemal distance between maternal and fetal circulation. Overall, our findings, that trophoblast\u2010specific increased expression of\n                    <jats:italic>hIGF1<\/jats:italic>\n                    results in changes to glucose transporter expression and increases fetal glucose concentrations within a short time period, highlights the translational potential this treatment could have in correcting impaired placental nutrient transport in human pregnancies complicated by FGR.\n                  <\/jats:p>","DOI":"10.1002\/mrd.23644","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T13:19:10Z","timestamp":1662988750000},"page":"540-553","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Nanoparticle\u2010mediated transgene expression of\n                    <i>insulin\u2010like growth factor 1<\/i>\n                    in the growth restricted guinea pig placenta increases placenta nutrient transporter expression and fetal glucose concentrations"],"prefix":"10.1002","volume":"89","author":[{"given":"Rebecca L.","family":"Wilson","sequence":"first","affiliation":[{"name":"Center for Research in Perinatal Outcomes University of Florida College of Medicine Gainesville Florida USA"},{"name":"Department of Physiology and Functional Genomics University of Florida College of Medicine Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Lampe","sequence":"additional","affiliation":[{"name":"Center for Fetal and Placental Research Cincinnati Children's Hospital and Medical Center Cincinnati Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukesh K.","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Vanderbilt University Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig L.","family":"Duvall","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Vanderbilt University Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen N.","family":"Jones","sequence":"additional","affiliation":[{"name":"Center for Research in Perinatal Outcomes University of Florida College of Medicine Gainesville Florida USA"},{"name":"Department of Physiology and Functional Genomics University of Florida College of Medicine Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140879"},{"issue":"2","key":"e_1_2_9_3_1","first-page":"7243","article-title":"Safety and efficacy of amine\u2010containing methacrylate polymers as nonviral gene delivery vectors","volume":"3","author":"Abd Ellah N. 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