{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T14:21:25Z","timestamp":1766067685365},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T00:00:00Z","timestamp":1672272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Preeclampsia (PE)\u00a0refers to a pregnancy\u2010specific disease that begins with the placenta. Differentially expressed microRNAs (miRs) are a feature of PE. This study tried to elicit the functional mechanism of miR\u2010515\u20105p in trophoblast cell behaviors in PE. First, HTR\u20108\/SVneo cells were transfected with miR\u2010515\u20105p mimic or miR\u2010515\u20105p inhibitor. Then, relative expression levels of miR\u2010515\u20105p and histone deacetylase 2 (HDAC2) in HTR\u20108\/SVneo cells were determined by reverse transcription\u2010quantitative polymerase chain reaction. The potential binding site of miR\u2010515\u20105p and HDAC2 was predicted on Targetscan and their binding relationship was verified via dual\u2010luciferase assay. Proliferation, apoptosis, invasion, and migration of HTR\u20108\/SVneo cells were assessed via cell counting kit\u20108, flow cytometry, Transwell, and wound healing assays, respectively. Protein levels of Cleaved caspase\u20103, Bcl\u20102, and Bax were determined via Western blot. Overexpressed miR\u2010515\u20105p impeded proliferation and stimulated apoptosis of HTR\u20108\/SVneo cells, and decreased levels of Cleaved caspase\u20103 and Bax and elevated Bcl\u20102, whilst opposite results were observed after miR\u2010515\u20105p inhibition. miR\u2010515\u20105p targeted HDAC2. Knockdown of HDAC2 annulled the promotional action of miR\u2010515\u20105p inhibition on proliferative, invasive, and migratory abilities and its antiapoptotic action on HTR\u20108\/SVneo cells. In brief, miR\u2010515\u20105p affected the proliferation, apoptosis, invasion, and migration of HTR\u20108\/SVneo cells by targeting HDAC2.<\/jats:p>","DOI":"10.1002\/mrd.23649","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T18:20:25Z","timestamp":1672338025000},"page":"59-66","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect and mechanism of microRNA\u2010515\u20105p in proliferation and apoptosis of trophoblast cells in preeclampsia via manipulating histone deacetylase 2"],"prefix":"10.1002","volume":"90","author":[{"given":"Ke","family":"Zhang","sequence":"first","affiliation":[{"name":"The Department of Obstetric The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailing","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Department of Obstetric The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Gao","sequence":"additional","affiliation":[{"name":"The Department of Obstetric The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caiping","family":"Sun","sequence":"additional","affiliation":[{"name":"The Department of Obstetric The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"The Department of Obstetric The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan 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