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P\u2010cadherin or Cdh3 is only expressed in Sertoli cells from embryonic to prepubertal development. Interestingly, the expression profile of <jats:italic>Cdh3<\/jats:italic> correlates with that of activating protein\u20101 (AP\u20101) transcription factors during Sertoli cells development. To assess their potential implications in the regulation of <jats:italic>Cdh3<\/jats:italic>, different AP\u20101 transcription factors were overexpressed in 15P\u20101 Sertoli cells. We found that the overexpressions of Junb and Fosl2 activated <jats:italic>Cdh3<\/jats:italic> promoter. ChIP\u2010qPCR assay and luciferase reporter assay with 5\u2032 promoter deletions and site\u2010directed mutagenesis confirmed the recruitment of Junb and Fosl2 to an AP\u20101 regulatory element at \u201047 bp in the proximal region of <jats:italic>Cdh3<\/jats:italic> promoter in 15P\u20101 cells. These findings were further supported by histone modification markers and chromatin accessibility surrounding <jats:italic>Cdh3<\/jats:italic> promoter in mouse testis. Moreover, the knockdowns of Junb and\/or Fosl2 by siRNA decreased Cdh3 protein levels. Taken together, these data suggest that in 15P\u20101 Sertoli cells, the AP\u20101 family members Junb and Fosl2 are responsible for the regulation of <jats:italic>Cdh3<\/jats:italic> expression, which requires the recruitment of both factors to the proximal region of the <jats:italic>Cdh3<\/jats:italic> promoter.<\/jats:p>","DOI":"10.1002\/mrd.23656","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T13:45:28Z","timestamp":1670247928000},"page":"27-41","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The transcription factors Junb and Fosl2 cooperate to regulate <i>Cdh3<\/i> expression in 15P\u20101 Sertoli cells"],"prefix":"10.1002","volume":"90","author":[{"given":"Ha T.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Biology Universit\u00e9 de Moncton  Moncton New Brunswick 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