{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:21:32Z","timestamp":1792081292655,"version":"4.4.0"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate possible causes of reproductive failure, we conducted global endometrial gene expression analyses in fertile and subfertile cows. Ingenuity pathway analysis showed that RICTOR and SIRT3 are significant upstream regulators for highly expressed genes in fertile cows, and are predicted to be activated upstream regulators of normal mitochondrial respiration. Canonical pathway analysis revealed that these highly expressed genes are involved in the activation of mitochondrial oxidative phosphorylation. Therefore, in subfertile cows, the inactivation of RICTOR and SIRT3 may correlate with decreased capacity of mitochondrial respiration. Furthermore, the expression levels of most mitochondrial DNA genes and nuclear genes encoding mitochondrial proteins were higher in subfertile cows. The mitochondrial DNA copy number was significantly higher in the endometrium of subfertile cows, whereas the ATP content did not differ between fertile and subfertile cows. Quantitative reverse transcription\u2010PCR\u00a0analysis demonstrated that the expression of <jats:italic>PGC1a, TFAM, MFN1, FIS1<\/jats:italic>, and <jats:italic>BCL2L13<\/jats:italic> were significantly lower in subfertile cows. In addition, transmission electron microscopy images showed mitochondrial swelling in the endometrial cells of the subfertile cow. These results suggest that poor\u2010quality mitochondria accumulate in the endometrium owing to a reduced capacity for mitochondrial biogenesis, fusion, fission, and degradation in subfertile cows, and may contribute to infertility.<\/jats:p>","DOI":"10.1002\/mrd.23670","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T20:03:20Z","timestamp":1673899400000},"page":"141-152","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Deterioration of mitochondrial biogenesis and degradation in the endometrium is a cause of subfertility in cows"],"prefix":"10.1002","volume":"90","author":[{"given":"Shuichi","family":"Matsuyama","sequence":"first","affiliation":[{"name":"Division of Animal Feeding and Management Research, Institute of Livestock and Grassland Science National Agriculture and Food Research Organization (NARO) Nasushiobara Japan"},{"name":"Laboratory of Animal Production Science, Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Division of Animal Feeding and Management Research, Institute of Livestock and Grassland Science National Agriculture and Food Research Organization (NARO) Nasushiobara Japan"},{"name":"Faculty of Veterinary Medicine Okayama University of Science  Imabari Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiori","family":"Minabe","sequence":"additional","affiliation":[{"name":"Division of Animal Feeding and Management Research, Institute of Livestock and Grassland Science National Agriculture and Food Research Organization (NARO) Nasushiobara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Sakatani","sequence":"additional","affiliation":[{"name":"Division of Animal Feeding and Management Research, Institute of Livestock and Grassland Science National Agriculture and Food Research Organization (NARO) Nasushiobara Japan"},{"name":"Livestock and Grassland Research Division, Kyushu Okinawa Agricultural Research Center National Agriculture and Food Research Organization (NARO) Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Takenouchi","sequence":"additional","affiliation":[{"name":"Livestock and Grassland Research Division, Kyushu Okinawa Agricultural Research Center National Agriculture and Food Research Organization (NARO) Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Production Science, Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan"},{"name":"Okayama A.I. 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