{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:10:49Z","timestamp":1772118649581,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T00:00:00Z","timestamp":1677715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Luteal dysfunctions lead to fertility disorders and pregnancy complications. Normal luteal function is regulated by many factors, including luteinizing hormone (LH).\u00a0The luteotropic roles of LH have been widely investigated but its role in the process of luteolysis has received little attention. LH has been shown to have luteolytic effects during pregnancy in rats and the role of intraluteal prostaglandins (PGs)\u00a0in LH\u2010mediated luteolysis has been demonstrated by others. However, the status of PG signaling in the uterus during LH\u2010mediated luteolysis remains unexplored. In this study, we utilized the repeated LH administration (4\u00d7LH)\u00a0model for luteolysis induction. We have examined the effect of LH\u2010mediated luteolysis on the expression of genes involved in luteal\/uterine PG synthesis, luteal PGF\n                    <jats:sub>2\u03b1<\/jats:sub>\n                    signaling, and uterine activation during different stages (mid and late) of pregnancy. Further, we analyzed the effect of overall PG synthesis machinery blockage on LH\u2010mediated luteolysis during late pregnancy. Unlike the midstage of pregnancy, the expression of genes involved in PG synthesis, PGF\n                    <jats:sub>2\u03b1<\/jats:sub>\n                    signaling, and uterine activation in late\u2010stage pregnant rats' luteal and uterine tissue increase 4\u00d7LH. Since the cAMP\/PKA\u00a0pathway mediates LH\u2010mediated luteolysis, we analyzed the effect of inhibition of endogenous PG synthesis on the cAMP\/PKA\/CREB\u00a0pathway, followed by the analysis of the expression of markers of luteolysis. Inhibition of endogenous PG synthesis did not affect the cAMP\/PKA\/CREB\u00a0pathway. However, in the absence of endogenous PGs, luteolysis could not be activated to the full extent. Our results suggest that endogenous PGs may contribute to LH\u2010mediated luteolysis, but this dependency on endogenous PGs is pregnancy\u2010stage dependent. These findings advance our understanding of the molecular pathways that regulate luteolysis.\n                  <\/jats:p>","DOI":"10.1002\/mrd.23678","type":"journal-article","created":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T02:41:35Z","timestamp":1677811295000},"page":"260-271","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Role of intraluteal and intrauterine prostaglandin signaling in LH\u2010induced luteolysis in pregnant rats"],"prefix":"10.1002","volume":"90","author":[{"given":"Akshi","family":"Vashistha","sequence":"first","affiliation":[{"name":"Department of Molecular Reproduction, Development and Genetics Indian Institute of Science Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Habibur Rahaman","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Molecular Reproduction, Development and Genetics Indian Institute of Science Bangalore India"},{"name":"Department of Safety Assessment Adgyl LifeSciences Private Limited Bangalore Karnataka"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/2045-3701-3-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gah158"},{"key":"e_1_2_8_4_1","first-page":"577","article-title":"P\u2010glycoprotein expression is increased in human secretory and gestational endometrium","volume":"65","author":"Axiotis C. 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