{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:38:49Z","timestamp":1791837529082,"version":"4.3.4"},"reference-count":31,"publisher":"Wiley","issue":"8-9","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pre\u2010eclampsia (PE) is usually defined as new\u2010onset hypertension with albuminuria or other organ damage. Herein, the role and mechanism of long noncoding RNA (lncRNA) gastric carcinoma high expressed transcript 1 (GHET1) during PE are investigated. Expression of GHET1 in PE pregnancies was evaluated using quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR). Proliferation and cell cycle of extravillous trophoblasts were assessed by Cell Counting Kit\u20108 (CCK\u20108), colony formation, 5\u2010Ethynyl\u20102\u2032\u2010deoxyuridine (EdU) assays, and flow cytometry, respectively. Migration, invasion, and network formation of trophoblasts were measured by wound healing, transwell system, and tube formation assays. RNA immunoprecipitation (RIP), RNA pull\u2010down, and chromatin immunoprecipitation (ChIP) assays were used to confirm the molecular interaction. GHET1 was markedly decreased in the placenta of PE patients. GHET1 promoted the proliferation and cell cycle of extravillous trophoblasts, as well as migration, invasion, and network formation in vitro. Metallothionein 2A (MT2A) functioned as a downstream effector of GHET1, which was negatively correlated with GHET1 in PE. GHET1 directly bound with zeste 2 polycomb repressive complex 2\/lysine\u2010specific demethylase 1 (EZH2\/LSD1). Knockdown of GHET1 reduced the occupancies of H3K27me3 and H3K4me2 in the MT2A promoter region by recruiting EZH2 and LSD1. MT2A knockdown reversed GHET1 inhibition mediated biological functions. GHET1 regulates extravillous trophoblastic phenotype via EZH2\/LSD1\u2010mediated MT2A epigenetic suppression in PE.<\/jats:p>","DOI":"10.1002\/mrd.23693","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T11:40:57Z","timestamp":1691408457000},"page":"758-770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["lncRNA GHET1 regulates extravillous trophoblastic phenotype via EZH2\/LSD1\u2010mediated MT2A epigenetic suppression in pre\u2010eclampsia"],"prefix":"10.1002","volume":"90","author":[{"given":"Pengyun","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Huang","sequence":"additional","affiliation":[{"name":"Reproductive Health Department Jiangxi Provincial Maternal and Child Health Hospital  Nanchang Jiangxi Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenting","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Su","sequence":"additional","affiliation":[{"name":"Pathology Department Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Traditional Chinese Medicine Department Duchang County People's Hospital  Jiujiang Jiangxi Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianggang","family":"Wang","sequence":"additional","affiliation":[{"name":"Medical Examination Department Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi Province People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.i1753"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/oncsis.2016.84"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3480"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2019.12.274"},{"issue":"1","key":"e_1_2_11_6_1","first-page":"31","article-title":"lncRNA GHET1 knockdown suppresses breast cancer activity in vitro and in vivo","volume":"11","author":"Han M.","year":"2019","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000003581"},{"issue":"2","key":"e_1_2_11_8_1","first-page":"1451","article-title":"lncRNA FAM99A is downregulated in preeclampsia and exerts a regulatory effect on trophoblast cell invasion, migration and apoptosis","volume":"20","author":"He T.","year":"2019","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2017.01.017"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1384-9"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S188396"},{"issue":"24","key":"e_1_2_11_12_1","first-page":"8561","article-title":"LncRNA HOXD\u2010AS1 promotes preeclampsia progression via MAPK pathway","volume":"22","author":"Jiang J.","year":"2018","journal-title":"European Review for Medical and Pharmacological Sciences"},{"issue":"5","key":"e_1_2_11_13_1","first-page":"1216","article-title":"LncRNA CCAT1 promotes the progression of preeclampsia by regulating CDK4","volume":"22","author":"Li J. 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