{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:05:06Z","timestamp":1704326706381},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T00:00:00Z","timestamp":1700611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In mammals, the generation of sperm cells capable of fertilization is a highly complex process including spermatogenesis in the testis and maturation in the epididymis. In our previous study, we have demonstrated that FAM71D (Family with sequence similarity 71, member D), which could interact with calmodulin, was highly expressed in human and mouse testis. To investigate the physiological role of FAM71D in spermatogenesis, we next generate\u00a0<jats:italic>Fam71d<\/jats:italic> loss\u2010of\u2010function mouse model using CRISPR\/Cas9 technology. We performed immunofluorescence and RT\u2010qPCR to examine the protein and mRNA expression in testicular cells. We found that FAM71D was predominantly localized in the round and elongated spermatids. And FAM71D KO mice displayed normal development of germ cell and fertility. Furthermore, testicular histology and sperm concentration showed no significant difference between WT and KO mice. These data demonstrate that FAM71D is dispensable for mouse spermatogenesis and male fertility.<\/jats:p>","DOI":"10.1002\/mrd.23716","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T05:35:38Z","timestamp":1700717738000},"page":"804-809","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["FAM71D is dispensable for spermatogenesis and male fertility in mice"],"prefix":"10.1002","volume":"90","author":[{"given":"Shaomei","family":"Mo","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Department of Urology Peking University Shenzhen Hospital, Clinical College of Anhui Medical University Shenzhen Guangdong China"},{"name":"The Fifth Clinical Medical College of Anhui Medical University Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keming","family":"Deng","sequence":"additional","affiliation":[{"name":"The Second Clinical Medical College Lanzhou University Lanzhou Gansu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congcong","family":"Cao","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Department of Urology Peking University Shenzhen Hospital, Clinical College of Anhui Medical University Shenzhen Guangdong China"},{"name":"The Fifth Clinical Medical College of Anhui Medical University Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoting","family":"Gui","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Department of Urology Peking University Shenzhen Hospital, Clinical College of Anhui Medical University Shenzhen Guangdong China"},{"name":"The Fifth Clinical Medical College of Anhui Medical University Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Ma","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Department of Urology Peking University Shenzhen Hospital, Clinical College of Anhui Medical University Shenzhen Guangdong China"},{"name":"The Fifth Clinical Medical College of Anhui Medical University Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei126"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2013.11.017"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1972.52.1.198"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmv020"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dml013"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh870"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585-018-0003-3"},{"key":"e_1_2_10_9_1","first-page":"273","article-title":"Characteristics of testis\u2010specific phosphoglycerate kinase 2 and its association with human sperm quality","volume":"31","author":"Liu X. 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