{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T20:07:31Z","timestamp":1770494851244,"version":"3.49.0"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Sodium Glucose Cotransporter Isoform 1 (Sglt\u20101) is a symporter that moves Na<jats:sup>+<\/jats:sup> and glucose into the cell. While most studies have focused on the role of Sglt\u20101 in the small intestine and kidney, little is known about this transporter's expression and function in other tissues. We have previously shown that Sglt\u20101 is expressed in the mouse sperm flagellum and that its inhibition interferes with sperm metabolism and function. Here, we further investigated the importance of Sglt\u20101 in sperm, using a Sglt\u20101 knockout mouse (Sglt\u20101 KO). RNA, immunocytochemistry, and glucose uptake analysis confirmed the ablation of Sglt\u20101 in sperm. Sglt\u20101 KO male mice are fertile and exhibit normal sperm counts and morphology. However, Sglt\u20101 null sperm displayed a significant reduction in total, progressive and other parameters of sperm motility compared to wild type (WT) sperm. The reduction in motility was exacerbated when sperm were challenged to swim in media with higher viscosity. Parameters of capacitation, namely protein tyrosine phosphorylation and acrosomal reaction, were similar in Sglt\u20101 KO and WT sperm. However, Sglt\u20101 KO sperm displayed a significant decrease in hyperactivation. The impaired motility of Sglt\u20101 null sperm was observed in media containing glucose as the only energy substrate. Interestingly, the addition of pyruvate and lactate to the media partially recovered sperm motility of Sglt\u20101 KO sperm, both in the low and high viscosity media. Altogether, these results support an important role for Sglt\u20101 in sperm energetics and function, providing sperm with a higher capacity for glucose uptake.<\/jats:p>","DOI":"10.1002\/mrd.23723","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T04:54:40Z","timestamp":1706072080000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Deletion of the Sodium Glucose Cotransporter 1 (Sglt\u20101) impairs mouse sperm movement"],"prefix":"10.1002","volume":"91","author":[{"given":"September","family":"Numata","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Physiology University of Kansas Medical Center  Kansas City Kansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mumtarin Jannat","family":"Oishee","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology University of Kansas Medical Center  Kansas 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