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Thus, the objective was to evaluate the SP\u00a0proteome in boars classified by capacity of semen preservation and putative biomarkers for fertility. The ejaculates from high\u2010preservation (HP) showed higher progressive motility during all 5 days than the low\u2010preservation (LP) boars. There was no difference for farrowing rate between ejaculates from LP (89.7%) and HP boars (88.4%). The LP boars presented lower total piglets born (14.0\u2009\u00b1\u20090.2) than HP (14.8\u2009\u00b1\u20090.2; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). A total of 257 proteins were identified, where 184 were present in both classes of boar, and 41 and 32 were identified only in LP and HP boars, respectively. Nine proteins were differently expressed: five were more abundant in HP (SPMI, ZPBP1, FN1, HPX, and C3) and four in LP boars (B2M, COL1A1, NKX3\u20102, and MPZL1). The HP boars had an increased abundance of SP proteins related to sperm resistance and fecundation process which explains the better TPB. LP boars had a higher abundance of SP proteins associated with impaired spermatogenesis.<\/jats:p>","DOI":"10.1002\/mrd.23735","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T04:38:58Z","timestamp":1706503138000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Proteomic analysis of boar seminal plasma: Putative markers for fertility based on capacity of semen preservation at 17\u00b0C"],"prefix":"10.1002","volume":"91","author":[{"given":"Matheus S.","family":"Lucca","sequence":"first","affiliation":[{"name":"Departamento de Medicina Animal Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul\u2014UFRGS, Setor de Su\u00ednos  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0793-8443","authenticated-orcid":false,"given":"Ivan C.","family":"Bustamante\u2010Filho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia Universidade do Vale do Taquari\u2010Univates  Lajeado Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael R.","family":"Ulguim","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Animal Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul\u2014UFRGS, Setor de Su\u00ednos  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael D. 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