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However, the mechanisms by which they are taken up by the follicular granulosa cells remain unclear. In addition, while oocytes play a pivotal role in follicular development, the possible interactions between oocyte\u2010derived paracrine factors (ODPFs) and EV signals are unknown. Therefore, this study aimed to elucidate the mechanism of EV uptake and the effects of ODPFs on EV uptake by follicular somatic mural granulosa cells in mice. Fluorescence\u2010labeled transferrin (TRF) and cholera toxin B (CTB), substrates for clathrin\u2010 and caveolae\u2010mediated endocytosis, respectively, were taken up by mural granulosa cells in vitro. Their uptake was inhibited by Pitstop 2 and genistein, inhibitors of clathrin and caveolae pathways, respectively. Mural granulosa cells took up EVs, and this uptake was suppressed by Pitstop 2 and genistein. Moreover, ODPFs promoted the uptake of EVs and CTB, but not TRF, by mural granulosa cells. These results suggest that mural granulosa cells take up EVs through both clathrin\u2010 and caveolae\u2010mediated endocytosis and that oocytes may promote caveolae\u2010mediated endocytosis to facilitate the uptake of EVs.<\/jats:p>","DOI":"10.1002\/mrd.23737","type":"journal-article","created":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T11:08:14Z","timestamp":1709809694000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Dynamics of extracellular vesicle uptake by mural granulosa cells in mice"],"prefix":"10.1002","volume":"91","author":[{"given":"Taichi","family":"Edure","sequence":"first","affiliation":[{"name":"Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo 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