{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:02:54Z","timestamp":1753869774661,"version":"3.41.2"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T00:00:00Z","timestamp":1716508800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this study was to investigate the impact of sperm source on embryo morphokinetics and the clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles by considering the clustering of data (multiple embryos per patient that share a comparable developmental timing). This matched cohort study was performed at a private university\u2013affiliated in vitro fertilization center. Women who underwent ICSI with epididymal sperm between January 2019 and December 2020 (the percutaneous epididymal sperm aspiration group, <jats:italic>n<\/jats:italic>\u2009=\u200932 cycles) were matched with women who underwent ICSI with ejaculated sperm because of idiopathic male factor infertility (the male factor infertility [MFI] group, <jats:italic>n<\/jats:italic>\u2009=\u200932 cycles) or female infertility (the control group, <jats:italic>n<\/jats:italic>\u2009=\u200932 cycles). Embryos were cultured in a time\u2010lapse imaging incubator, and morphokinetic development was recorded and compared among the groups. Significantly slower divisions were observed in embryos derived from epididymal sperm than in those derived from the MFI and control groups. Embryos derived from epididymal sperm had a significantly lower KIDScore (3.1\u2009\u00b1\u20090.2) than did those derived from ejaculated spermatozoa from the MFI (5.4\u2009\u00b1\u20090.1) and control (5.6\u2009\u00b1\u20090.2, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) groups. Epididymal sperm\u2010derived embryos showed a significantly greater occurrence of multinucleation (23.2%) than did those derived from ejaculated sperm from the MFI and control groups (2.8% and 3.7%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001, respectively). Epididymal sperm\u2010derived embryos were significantly more likely to undergo direct or reverse cleavage (11.1%) than ejaculated sperm\u2010derived embryos in the control group (4.3%, <jats:italic>p<\/jats:italic>\u2009=\u20090.001). In conclusion, delayed cell cleavage and increased incidences of blastomere multinucleation and abnormal cleavage patterns are observed when epididymal\u2010derived sperm are used for ICSI.<\/jats:p>","DOI":"10.1002\/mrd.23747","type":"journal-article","created":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T11:29:35Z","timestamp":1716550175000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Association between embryo morphokinetic development and intracytoplasmic sperm injection with epididymal sperm via time\u2010lapse imaging"],"prefix":"10.1002","volume":"91","author":[{"suffix":"Jr.","given":"Edson","family":"Borges","sequence":"first","affiliation":[{"name":"Fertility Medical Group S\u00e3o Paulo City S\u00e3o Paulo Brazil"},{"name":"Department of Scientific Research Instituto Sapientiae S\u00e3o Paulo City S\u00e3o Paulo 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