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However, it is unknown whether there are other mechanisms by which CABS1 affects male fertility. Our current investigation has uncovered that CABS1 is located in the midsection of the flagellum in testicular sperm and the principal piece in epididymal sperm. Moreover, male mice lacking CABS1 exhibit a defect in the progressive motility of sperm. Furthermore, the regulation of calcium levels, which has been reported to have a significant impact on sperm motility, capacitation, and the acrosome reaction, is also affected when sperm are exposed to alkalized high\u2010salt buffer (pH 8.0) and progesterone (100\u2009\u03bcM) in Cabs1\u2010null spermatozoa. This alteration in calcium response may contribute to changes in the phosphorylation of PKA substrates and subsequent phosphorylation of tyrosine residues. Additionally, the absence of CABS1 leads to a defective fibrous sheath and abnormal configuration of doublet microtubules in post\u2010testicular sperm. These findings indicate that the absence of CABS1 also disrupts the structural integrity of the fibrous sheath, resulting in male subfertility. The highly conserved nature of CABS1 in humans suggests that it could potentially be a contributing factor to asthenozoospermia in men.<\/jats:p>","DOI":"10.1002\/mrd.23776","type":"journal-article","created":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T11:44:55Z","timestamp":1731325495000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["CABS1 Is Essential for Progressive Motility and the Integrity of Fibrous Sheath in Mouse Epididymal Spermatozoa"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1483-3981","authenticated-orcid":false,"given":"Xiuling","family":"Zhao","sequence":"first","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School Nantong University Nantong Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School Nantong University Nantong Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyu","family":"Nie","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School Nantong University Nantong Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School Nantong University Nantong Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuhui","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School Nantong University Nantong Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Center of Reproductive Medicine, Affiliated Hospital of Nantong University  Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2795(200012)57:4<384::AID-MRD11>3.0.CO;2-U"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1260183"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.072535kb"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2011-4311"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1988.tb01007.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900082m"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011252200"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-015-2320-7"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioad076"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00612"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10320"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400050101"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb-nm-fertilityS57"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.112.105262"},{"issue":"1","key":"e_1_2_9_16_1","first-page":"2","article-title":"Signaling Mechanisms in Mammalian Sperm Motility","volume":"96","author":"Freitas M. 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