{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:37:47Z","timestamp":1789699067937,"version":"4.0.1"},"reference-count":80,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:00:00Z","timestamp":1739059200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ovarian tissue transplantation (OTT) has been suggested as an alternative to preserving female fertility in livestock species, as currently performed in women. The OTT technique has been tested as xenografting or autografting in different body sites and animal species. Currently, there are no reports available regarding the autotransplantation of ovarian tissue using the bovine model and also testing the effect of vascular endothelial growth factor (VEGF) on graft survival. This study evaluated the effects of ovarian tissue short\u2010term exposure to VEGF before heterotopic autotransplantation into a subcutaneous site (flank region) for 7 days in cattle. The initial finding was that after OTT and without pre\u2010exposure to VEGF, the ovarian grafts had 42% of normal preantral follicles, which indicates a substantial step forward for this technique in cattle. Furthermore, VEGF exposure actively facilitated the process of neoangiogenesis, the proliferation capability of the stromal cells for activation of the cell cycle, maintained the balance between types I and III collagen fibers, and reduced the total collagen of the grafted tissue. Moreover, ovarian fragments previously exposed to VEGF tended to have greater follicular density; however, a detrimental effect of VEGF on follicular morphology was noticed. In conclusion, this study marks a significant step forward in bovine OTT and provides a foundation for further investigations into the specific pathways, stages, and durations of VEGF exposure to unveil strategies for refining ovarian transplantation techniques in cattle and other species.<\/jats:p>","DOI":"10.1002\/mrd.70009","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T12:44:40Z","timestamp":1739277880000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Short\u2010Term Bovine Ovarian Tissue Heterotopic Autotransplantation: VEGF Beneficial and Detrimental Effects"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8304-4644","authenticated-orcid":false,"given":"Ana N. P.","family":"Morais","sequence":"first","affiliation":[{"name":"Laboratory of Manipulation of Oocytes and Preantral Follicles Faculty of Veterinary Medicine, State University of Cear\u00e1 Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5129-940X","authenticated-orcid":false,"given":"Samara S.","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Diagnostic Imaging Applied to Animal Reproduction Faculty of Veterinary Medicine, State University of Cear\u00e1 Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4550-0714","authenticated-orcid":false,"given":"Francisco L. N.","family":"Aguiar","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Sousa Campus, Federal Institute of Education, Science and Technology of Para\u00edba Sousa Para\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5317-2207","authenticated-orcid":false,"given":"Gustavo D. A.","family":"Gastal","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investigaci\u00f3n Agropecuaria, Estaci\u00f3n Experimental INIA La Estanzuela Colonia Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8244-2432","authenticated-orcid":false,"given":"Fabiana A. S.","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Diagnostic Imaging Applied to Animal Reproduction Faculty of Veterinary Medicine, State University of Cear\u00e1 Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8883-6914","authenticated-orcid":false,"given":"Juliany A.","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Sousa Campus, Federal Institute of Education, Science and Technology of Para\u00edba Sousa Para\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0085-7306","authenticated-orcid":false,"given":"Lucy V. S.","family":"\u00d1aupas","sequence":"additional","affiliation":[{"name":"Laboratory of Manipulation of Oocytes and Preantral Follicles Faculty of Veterinary Medicine, State University of Cear\u00e1 Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7359-3570","authenticated-orcid":false,"given":"Kele A.","family":"Alves","sequence":"additional","affiliation":[{"name":"Laboratory of Manipulation of Oocytes and Preantral Follicles Faculty of Veterinary Medicine, State University of Cear\u00e1 Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9465-8054","authenticated-orcid":false,"given":"Benner G.","family":"Alves","sequence":"additional","affiliation":[{"name":"Laboratory of Manipulation of Oocytes and Preantral Follicles Faculty of Veterinary Medicine, State University of Cear\u00e1 Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0043-0137","authenticated-orcid":false,"given":"Melba O.","family":"Gastal","sequence":"additional","affiliation":[{"name":"Animal Science, School of Agricultural Sciences Southern Illinois University Carbondale Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9786-1250","authenticated-orcid":false,"given":"Ana P. R.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Manipulation of Oocytes and Preantral Follicles Faculty of Veterinary Medicine, State University of Cear\u00e1 Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5139-0964","authenticated-orcid":false,"given":"Jos\u00e9 R.","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Laboratory of Manipulation of Oocytes and Preantral Follicles Faculty of Veterinary Medicine, State University of Cear\u00e1 Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1799-1675","authenticated-orcid":false,"given":"D\u00e1rcio \u00cd. A.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laboratory of Diagnostic Imaging Applied to Animal Reproduction Faculty of Veterinary Medicine, State University of Cear\u00e1 Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8855-6980","authenticated-orcid":false,"given":"Eduardo L.","family":"Gastal","sequence":"additional","affiliation":[{"name":"Animal Science, School of Agricultural Sciences Southern Illinois University Carbondale Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2007.00915.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.01.011"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.04.045"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2021.05.015"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0149693"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.12.004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/children9081256"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.08.004"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2019.03.003"},{"issue":"14","key":"e_1_2_9_11_1","first-page":"4123","article-title":"VEGF165b, an Inhibitory Splice Variant of Vascular Endothelial Growth Factor, Is Down\u2010Regulated in Renal Cell Carcinoma","volume":"62","author":"Bates D. O.","year":"2002","journal-title":"Cancer Research"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-12-0121"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-023-10139-5"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21041388"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-03167-6"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.06.029"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deq.131"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2021.03.008"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.197384"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD17315"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1017\/s0967199417000338"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2014.09.003"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2009.09.003"},{"key":"e_1_2_9_24_1","first-page":"400","article-title":"Body Condition Scoring Beef Cows","author":"Eversole D. E.","year":"2009","journal-title":"Virginia Cooperative Extension"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-017-1051-y"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehr304"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep-16-0621"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.4045\/tidsskr.21.0030"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.11.012"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajh.2012.17"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2019.04.002"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.1994.9694423"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-022-01048-0"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2013.0517"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc1950.41.267"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-015-0628-6"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0184546"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/tp.0b013e318279965c"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2016.02.029"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-021-00517-2"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17081237"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01460-1"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/1179558119867357"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.574276"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2021.100575"},{"key":"e_1_2_9_46_1","volume-title":"Nutrient Requirements of Beef Cattle","author":"National Academies of Sciences, Engineering, and Medicine","year":"2016"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-021-00593-4"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2022.105123"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms151119791"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2007.05.069"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2021.08.034"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2021.01.005"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25934-4"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4bm00141a"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2020.106310"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.2196\/12944"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2009.11.015"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.2527\/1998.7661671x"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431x20187129"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1071\/rd14454"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-018-4180-5"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12081575"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-021-02529-w"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.21037\/gs-21-24"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology10111212"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065x.2006.00434.x"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1530\/raf-21-0008"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0241442"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medengphy.2006.07.010"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.4103\/2008-7802.104853"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1080\/08977194.2016.1194835"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-022-00910-9"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.22074\/ijfs.2020.134678"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2018.09.010"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-56163-x"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1159\/000322399"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-013-0043-9"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2012.02.025"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719107309818"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2007.08.086"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.4274\/jtgga.2017.0026"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.70009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T09:51:38Z","timestamp":1741773098000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.70009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":80,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/mrd.70009"],"URL":"https:\/\/doi.org\/10.1002\/mrd.70009","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-05-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70009"}}