{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:02:53Z","timestamp":1753869773966,"version":"3.41.2"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A role for the plasminogen activator (PA) system has been postulated in mammalian gonads, considering the complex process of morphogenesis these organs undergo during their development. Our results show that mouse Sertoli cells under basal conditions produce both types of PA, tissue\u2010type PA (tPA) and urokinase\u2010type PA (uPA), and hormonal treatments increase the production of both enzymes. The increased enzyme secretion does not correlate with a parallel increase in their mRNAs. However, the proteolytic activity results from a balance between enzyme activity and inhibitors. Hormonal stimulation decreased the expression of the inhibitor PAI\u20101, suggesting that the increase in proteolytic activity might depend on the decreased production of PAI\u20101.<\/jats:p><jats:p>The expression of the two enzymes and their inhibitor depends on the seminiferous epithelium stage. We observed higher <jats:italic>uPA<\/jats:italic> mRNA levels at stages VII\u2010VIII and IX\u2010XII, <jats:italic>tPA<\/jats:italic> peaks at stages VII\u2010VIII, and <jats:italic>PAI\u20101<\/jats:italic> mRNA levels decreased at stages VII\u2010VIII and IX\u2010XII.<\/jats:p><jats:p>The testes from mice lacking the <jats:italic>uPA<\/jats:italic> gene (<jats:italic>uPA<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>) presented statistically smaller sizes and weights. Histological analysis of <jats:italic>uPA<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>animals showed tubular morphology defects and atypical residual bodies (RB), suggesting a defect in Sertoli cell phagocytosis. Moreover, we show lower sperm concentration and motility in <jats:italic>uPA<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice. These data suggested an effective deficiency of testicular development in the absence of uPA.<\/jats:p>","DOI":"10.1002\/mrd.70012","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T09:14:27Z","timestamp":1738055667000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hormonal Regulation of Urokinase\u2010 and Tissue\u2010Type Plasminogen Activator in Mouse Sertoli Cells"],"prefix":"10.1002","volume":"92","author":[{"given":"Sara","family":"Carosi","sequence":"first","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Section of Histology Sapienza University of Rome Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Innocenti","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Section of Histology Sapienza University of Rome Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Monaco","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Sapienza University of Rome Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaia","family":"Laurenzi","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Section of Histology Sapienza University of Rome Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossana","family":"Saracino","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Section of Histology Sapienza University of Rome Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Canipari","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Section of Histology Sapienza University of Rome Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0399-0145","authenticated-orcid":false,"given":"Elena","family":"Vicini","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Section of Histology Sapienza University of Rome Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-6-2613"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-124-3-1399"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(90)90164-4"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0484fje"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00293.2012"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950150206"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.2.544"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.2.977"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89187-6"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-4-1652"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/368419a0"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.07.050"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines3010089"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21400"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60028-7"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1160\/th05-01-0054"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.133.1.8319585"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/endocrines3030037"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.03.071"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.78.1.8288701"},{"key":"e_1_2_9_22_1","first-page":"217","volume-title":"The Sertoli Cell","author":"Fritz I. 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