{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T23:22:47Z","timestamp":1767136967677,"version":"build-2238731810"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The functional role of estrogen receptor activation factor (E\u2010RAF), a 66\u2009kDa protein in mediating the biological actions of estrogen and progesterone in goat uterus, has been highlighted in this work. Here we report that E\u2010RAF stimulates the expression of splicing factor gene, arginine\/serine rich in goat uterine nuclei (GenBank Accession No. MT473439). cDNA subtractive hybridization method using nuclear run\u2010on transcription assay was used to identify the differentially expressed genes. The upregulation of the splicing factor gene was demonstrated further using a PCR\u2010based nuclear run\u2010on transcription system. Confocal microscopic analysis has confirmed the endoplasmic reticulum as the primary site of intracellular location of E\u2010RAF. The effect of estrogen and progesterone on the intracellular distribution of E\u2010RAF and the molecular mechanisms that underlie the transport of E\u2010RAF to nucleus in the presence of progesterone has been studied. Our results offer a new perspective in steroid hormone action in uterus.<\/jats:p>","DOI":"10.1002\/mrd.70023","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T01:49:32Z","timestamp":1744163372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential Expression of Splicing Factor Gene by Estrogen Receptor Activation Factor"],"prefix":"10.1002","volume":"92","author":[{"given":"Julie","family":"Jacob","sequence":"first","affiliation":[{"name":"Molecular Endocrinology Laboratory Rajiv Gandhi Centre for Biotechnology Thiruvananthapuram Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vidya S.","family":"Krishnan","sequence":"additional","affiliation":[{"name":"Molecular Endocrinology Laboratory Rajiv Gandhi Centre for Biotechnology Thiruvananthapuram Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Sreeja","sequence":"additional","affiliation":[{"name":"Molecular Endocrinology Laboratory Rajiv Gandhi Centre for Biotechnology Thiruvananthapuram Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raghava Varman","family":"Thampan","sequence":"additional","affiliation":[{"name":"Molecular Endocrinology Laboratory Rajiv Gandhi Centre for Biotechnology Thiruvananthapuram Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2016-1654"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2018.11.038"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1994.1103"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2300465"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150080203"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.100"},{"key":"e_1_2_8_9_1","first-page":"68","volume-title":"Laboratory Techniques in Biochemistry and Molecular Biology","author":"Chard T.","year":"1987"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27987"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007269507856"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10483"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10482"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/01485017908985044"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.2.293"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(00)00132-4"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaab024"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(99)00052-0"},{"issue":"6","key":"e_1_2_8_19_1","first-page":"1207","article-title":"Estrogen Receptor Activation Factor (E\u2010RAF) of the Rat Uterus: Hormonal Control","volume":"37","author":"Kumar P.","year":"1995","journal-title":"Biochemistry and Molecular Biology International"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqad081"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20081501"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1000637"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(74)90139-0"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.20.3.0366"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4892(01)00105-9"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11565"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(00)00188-6"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-2243(94)90138-4"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/265069a0"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1910743"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.85.5.6537"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2017.11.576"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471143030.cb0404s00"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(87)90198-5"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(89)90061-0"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/290152a0"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(20000601)77:3<382::AID-JCB4>3.0.CO;2-L"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90024-5"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)36750-X"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(61)90291-0"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00403363"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22701"}],"updated-by":[{"DOI":"10.1002\/mrd.70031","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,5,11]],"date-time":"2025-05-11T00:00:00Z","timestamp":1746921600000}}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.70023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:11:15Z","timestamp":1745799075000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.70023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/mrd.70023"],"URL":"https:\/\/doi.org\/10.1002\/mrd.70023","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"2024-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70023"}}