{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T19:30:05Z","timestamp":1777145405481,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Hypoxia\u2010inducible factor 1\u2010alpha (HIF\u20101\u03b1) is essential for glycolysis regulation. Its expression in the endometrium is significantly reduced in recurrent implantation failure (RIF), indicating that lower levels of HIF\u20101\u03b1 may contribute to embryo implantation failure. O\u2010GlcNAcylation is a dynamic posttranslational modification mediated by O\u2010GlcNAc transferase (OGT), known to regulate HIF\u20101\u03b1 in cancer cells. However, it remains unclear whether OGT affects glycolytic processes in uterine endometrial stromal cells (ESCs) and its potential role in embryo implantation. This study utilized In Vitro and In Vivo experiments to investigate the role of OGT in decidualization and embryo implantation, along with its underlying mechanisms. Our findings show that OGT expression is significantly reduced in the endometrium of patients with RIF. Additionally, OGT knockdown led to failed embryo implantation in mice. Further analysis revealed that OGT promotes decidualization by stabilizing HIF\u20101\u03b1, which enhances glycolytic activity. Inhibiting OGT resulted in insufficient decidualization among human ESCs. Moreover, our results indicate that OGT partially regulates CCL2 secretion by maintaining HIF\u20101\u03b1 levels within human ESCs, which is essential for successful embryo implantation. Based on these findings, we propose that OGT represents a novel and promising therapeutic target for both the diagnosis and treatment of RIF.<\/jats:p>","DOI":"10.1002\/mrd.70025","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T11:49:07Z","timestamp":1746704947000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Decreased OGT Attenuates Endometrial Decidualization and Embryo Implantation by Affecting HIF\u20101\u03b1 Stability"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7818-0070","authenticated-orcid":false,"given":"Bo","family":"Li","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Tangdu Hospital The Fourth Military Medical University Xi'an 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