{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:02:57Z","timestamp":1753869777913,"version":"3.41.2"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T00:00:00Z","timestamp":1746316800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In this study, we examine the transcriptional profiles of eight stages of preimplantation embryo development, from germinal vesicle (GV) oocytes to blastocysts, in in vitro fertilized sheep embryos using RNA sequencing. Between 12,744 and 15,539 genes were detected from the GV moving forward to blastocyst, with cluster analysis revealing two distinct clusters related to developmental stage. The largest change for differentially expressed genes (DEGs) numbers took place between 2\u2010 to 4\u2010cell comparison and 4\u2010 to 8\u2010cell comparison, with a c. 35\u2010fold increase from 19 to 670 DEGs. This suggests that zygotic genome activation occurred at 8\u2010cell stage in sheep. All the DEGs had gene ontology annotations in three classes, and these comprised 53 subclasses. Most of the pathways (89 of 93) were enriched through KEGG analysis of the DEGs, and the most enriched pathway was oxidative phosphorylation. The 2\u2010 and 16\u2010cell stage embryos possessed the highest and lowest numbers, respectively, of single nucleotide polymorphisms and insertion\u2010deletion markers. Five major types of alternative splicing were found. A total of 2361 transcription factor genes were mutually expressed among the eight developmental stages. The top five differential transcription factor protein families were Zf\u2010C2H2, bHLH, Homeobox, Others, and HMG. This is the first study to investigate the transcriptional profiling of sheep preimplantation embryos at different developmental stages, and it displays a comprehensive transcriptome landscape in sheep oocytes and embryos.<\/jats:p>","DOI":"10.1002\/mrd.70028","type":"journal-article","created":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T12:53:33Z","timestamp":1746363213000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptome Analysis of Individual Ovine Oocytes and Preimplantation Embryos From In Vitro Fertilization Source"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9067-6562","authenticated-orcid":false,"given":"Hai\u2010Jun","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine Tianjin Academy of Agriculture Sciences Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang\u2010Yun","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Hebei Technology Innovation Center of Cattle and Sheep Embryo Hebei Agricultural University Baoding Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Jun","family":"Huang","sequence":"additional","affiliation":[{"name":"Service Center for Agricultural Development of Liaoning Province Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1768-322x.1986.tb00506.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/iox114"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0820769"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14127"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0860637"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-756"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12716"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.060426"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81769-9"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.08.040"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178041"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14122"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb08392.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg144"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/8.4.323"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/1984-3143-AR2023-0055"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2660"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.105562"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13546"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.709498"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.70028","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T07:15:41Z","timestamp":1748416541000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.70028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/mrd.70028"],"URL":"https:\/\/doi.org\/10.1002\/mrd.70028","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"type":"print","value":"1040-452X"},{"type":"electronic","value":"1098-2795"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2024-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70028"}}