{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:24:24Z","timestamp":1773321864720,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Recurrent pregnancy loss (RPL) is one of obstetrical diseases with no effective therapy methods. Trophoblast cell dysfunction and inflammation induce embryo implantation insufficiency, thereby resulting in RPL. OTU deubiquitinase with linear linkage specificity (OTULIN) plays a role in regulating the immune response and cell death. However, the role of OTULIN in RPL remains unclear. Spontaneous abortion mouse model and lipopolysaccharide\u2010treated HTR\u20108\/SVneo cells were used to investigate the role of OTULIN in RPL. OTULIN expression was downregulated in the labyrinth trophoblast of RPL mice and LPS\u2010treated trophoblast cells. The embryonic reabsorption rate was decreased in OTULIN\u2010overexpressed spontaneous abortion mice, accompanied with the increase in placental\/fetus weight ratio. OTULIN overexpression significantly inhibited apoptosis in vivo and in vitro, as evidenced by the decrease in the activity of caspase 3. The expression of pro\u2010inflammatory cytokines was decreased with OTULIN overexpression. Moreover, OTULIN overexpression decreased p\u2010I\u03baB\u03b1\/I\u03baB\u03b1 and p\u2010p65\/p65 ratio. The nuclear translocation of NF\u2010\u03baB was suppressed via OTULIN overexpression both in vivo and in vitro. Our study suggested that OTULIN deficiency might cause inflammation and trophoblast abnormalities in RPL. The supplementation with OTULIN might alleviate the development of RPL via inhibiting NF\u2010\u03baB mediated inflammation response.<\/jats:p>","DOI":"10.1002\/mrd.70029","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T17:10:41Z","timestamp":1747329041000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Upregulation of OTULIN Alleviated Recurrent Pregnancy Loss by Suppressing Trophoblast Dysfunction and NF\u2010\u03baB Signaling Pathway"],"prefix":"10.1002","volume":"92","author":[{"given":"Junmei","family":"Fan","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center, Shanxi Children's Hospital, Shanxi Women and Children Health Hospital Taiyuan Shanxi China"},{"name":"Reproductive Medicine Center, The affiliated Children's Hospital of Shanxi Medical University, Shanxi Women and Children Health Hospital Taiyuan Shanxi 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