{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T22:19:12Z","timestamp":1785190752430,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study assessed transcriptional changes in human cumulus cells (CCs) during oocyte maturation In Vitro. CCs were collected from 25 cumulus\u2010oocyte complexes derived from surplus ovarian medulla tissue of eight women (mean age 29 years, range 19\u201336) undergoing ovarian tissue cryopreservation without ovarian stimulation. Samples included CCs from fresh germinal vesicle (GV) oocytes (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095), and from GV (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20098) and metaphase II (MII) oocytes (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912) after 48 h of In Vitro maturation (IVM). Microarray analysis revealed active signaling pathways during IVM, emphasizing\n                    <jats:italic>LHCGR<\/jats:italic>\n                    upregulation as central to oocyte maturation. Enhanced pathways included the insulin\u2010like growth factor (IGF) system, particularly IGF2, and activin\/inhibin signaling, while others appeared less active In Vitro compared to In Vivo. Differential expression analysis identified 1763 significantly expressed genes (DEGs) between fresh GV and MII\u2010IVM, 50 DEGs between GV\u2010IVM and MII\u2010IVM, and 339 novel or unknown transcripts. Clustering highlighted additional pathways, such as MAPK, PPAR, Wnt, cholesterol metabolism, PI3K\u2010AKT, TGF\u2010\u03b2, focal adhesion, actin cytoskeleton regulation, and RANK\/RANKL, with differential regulation during IVM. These findings underscore the complexity of signaling in CCs and the distinct regulatory mechanisms of human oocyte maturation In Vitro compared to In Vivo.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70035","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T02:19:57Z","timestamp":1750126797000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Transcriptional Changes in Cumulus Cells During In Vitro Maturation of Human Oocytes"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8164-3697","authenticated-orcid":false,"given":"Jes\u00fas","family":"Cadenas","sequence":"first","affiliation":[{"name":"Laboratory of Reproductive Biology, The Juliane Marie Center for Women, Children, and Reproduction University Hospital of Copenhagen, Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Subiran","family":"Adrados","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Biology, The Juliane Marie Center for Women, Children, and Reproduction University Hospital of Copenhagen, Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stine Gry","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Biology, The Juliane Marie Center for Women, Children, and Reproduction University Hospital of Copenhagen, Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten Tryde","family":"Macklon","sequence":"additional","affiliation":[{"name":"The Fertility Clinic University Hospital of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinnie Hornsh\u00f8j","family":"Greve","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Aarhus University Hospital Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Fedder","sequence":"additional","affiliation":[{"name":"Centre of Andrology and Fertility Clinic Odense University Hospital Odense Denmark"},{"name":"Research Unit of Human Reproduction, Department of Clinical Research University of Southern Denmark Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linn Salto","family":"Mamsen","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Biology, The Juliane Marie Center for Women, Children, and Reproduction University Hospital of Copenhagen, Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus Yding","family":"Andersen","sequence":"additional","affiliation":[{"name":"The Fertility Clinic Copenhagen University Hospital, Herlev and Gentofte Hospital Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-018-01398-2"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deq049"},{"key":"e_1_2_10_4_1","unstructured":"Affymetrix Inc. 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