{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:40:38Z","timestamp":1791916838328,"version":"4.3.4"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,22]],"date-time":"2025-06-22T00:00:00Z","timestamp":1750550400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Oligoasthenospermia (OAS), a major\u00a0cause of male infertility, was alleviated by Guilu\u2010Erxian\u2010Glue (GLEXG) in\n                    <jats:italic>Tripterygium wilfordii polyglycoside<\/jats:italic>\n                    (TWG)\u2010induced rat models. This study identified GLEXG's bioactive ingredients and explored its therapeutic mechanism. Using network pharmacology and liquid chromatography\u2010mass spectrometry, quercetin was predicted and validated as a key component of GLEXG. In vivo and in vitro, OAS models were established using TWG in mice and H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    in spermatocytes. TWG administration reduced testicle and epididymis indices, sperm concentration, vitality, and serum testosterone, luteinizing hormone, and follicle\u2010stimulating hormone. It also\u00a0increased germ cell apoptosis, upregulated Bax, cleaved caspase\u20103,\u00a0and cleaved caspase\u20109, downregualted Bcl\u20102, PHB1, VDHC, SDHA, and CoxIV, and impaired mitochondrial function (reduced mitochondrial DNA copy number and membrane potential). Quercetin counteracted these effects in vivo and in vitro. Mechanistically, quercetin increased p\u2010PKA\/PKA and p\u2010CREB\/CREB ratios, upregulated Sirt1, and decreased Ac\u2010p53\/p53 ratio. These beneficial effects were abolished upon Sirt1 inhibition. Thus, quercetin in GLEXG ameliorated OAS by attenuating apoptosis and mitochondrial dysfunction via PKA\/CREB pathway\u2010dependent activation of Sirt1 and deacetylation of p53.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70036","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T02:23:56Z","timestamp":1750645436000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Bioactive Ingredient Quercetin in Guilu\u2010Erxian\u2010Glue Ameliorates Oligoasthenospermia in Mice via PKA\/CREB Pathway\u2010Dependent Sirt1 Activation and p53 Deacetylation"],"prefix":"10.1002","volume":"92","author":[{"given":"Yingqiu","family":"Li","sequence":"first","affiliation":[{"name":"Medical School Hunan University of Chinese Medicine Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianyu","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Acupuncture\u2010Moxibustion, Tuina and Rehabilitation Hunan University of Chinese Medicine Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minglian","family":"Wang","sequence":"additional","affiliation":[{"name":"Medical School Hunan University of Chinese Medicine Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxi","family":"Tang","sequence":"additional","affiliation":[{"name":"Medical School Hunan University of Chinese Medicine Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Du","sequence":"additional","affiliation":[{"name":"Medical School Hunan University of Chinese Medicine Changsha Hunan 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