{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:07:47Z","timestamp":1772986067617,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    c\u2010Abl encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell growth, proliferation, differentiation, division, and regulation of cytoskeletal structure. mTERT is a catalytic subunit of mouse telomerase and it is very important for controlling cell proliferation and homeostasis by maintaining telomere length. We demonstrated before the interaction between c\u2010Abl and mTERT in mouse ovary and we suggested a role for c\u2010Abl in the regulation of telomerase function and proliferation in mouse granulosa cells. The current study aims to examine the c\u2010Abl and mTERT expression and potential interactions through mouse preimplantation embryonic development. To assess c\u2010Abl's function in embryonic development, siRNA\u2010mediated silencing of the\n                    <jats:italic>c\u2010Abl<\/jats:italic>\n                    was used in mouse preimplantation embryos. After siRNA transfection, the immunofluorescence was used to examine the c\u2010Abl pattern at embryonic development. Next, the levels of\n                    <jats:italic>mTERT<\/jats:italic>\n                    and\n                    <jats:italic>c\u2010Abl<\/jats:italic>\n                    mRNA were compared. The results show that c\u2010Abl is expressed in mouse preimplantation embryos at all developmental stages, with the cytoplasmic expression all through from the 2\u2010cell to the blastocyst. Additionally, c\u2010Abl is presented very intense expression in blastomer\u2010blastomer junctions. The siRNA\u2010mediated depletion of\n                    <jats:italic>c\u2010Abl<\/jats:italic>\n                    showed developmental abnormalities at the 8\u2010 to 16\u2010cell and morula to blastocyst and also with significantly decreased blastocyst development rate. Moreover, expression of the mTERT telomerase catalytic subunit was significantly reduced in\n                    <jats:italic>c\u2010Abl<\/jats:italic>\n                    \u2010depleted embryos during preimplantation embryonic development. Finally, we demonstrate that c\u2010Abl may play a crucial role in compaction and preimplantation embryo development, and that the relationship between c\u2010Abl and mTERT has developmental importance in early embryogenesis.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70039","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T03:35:35Z","timestamp":1750217735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["c\u2010Abl Plays an Important Role in Mouse Preimplantation Embryo Development and the Dysregulation Associated With Decreased mTERT Expression"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0255-1442","authenticated-orcid":false,"given":"Ecem","family":"Yildirim","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology University of Health Sciences, Faculty of Medicine \u0130stanbul Turkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tugce","family":"Onel","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Demiroglu Bilim University Istanbul Turkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6781-9983","authenticated-orcid":false,"given":"Aylin","family":"Yaba","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Yeditepe University Faculty of Medicine \u0130stanbul T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1994.tb01118.x"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0925-4773(00)00423-8"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.06.023"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059528"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.earlhumdev.2010.08.024"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.earlhumdev.2010.06.002"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/350569a0"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2010.05.006"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1150017"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2007.09.031"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1007994"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.067702"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1280"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2003.11.003"},{"key":"e_1_2_13_16_1","first-page":"93","article-title":"In Vitro Development of Inner Cell Masses Isolated Immunosurgically From Mouse Blastocysts. 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