{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T21:51:31Z","timestamp":1784670691881,"version":"3.55.0"},"reference-count":72,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Although gamma\u2010aminobutyric acid (GABA) is known to play a role in the regulation of several physiological functions in vertebrates, the role of GABA in regulating the reproductive axis in viviparous teleosts remains opaque. This study examined the effects of GABA<jats:sub>A<\/jats:sub> and GABA<jats:sub>B<\/jats:sub> receptor agonists, muscimol (MUSC) and baclofen (BAC), on the hypothalamic\u2013pituitary\u2013ovarian (HPO) axis in the black molly <jats:italic>Poecilia sphenops<\/jats:italic>. A dose\u2010dependent upregulation of gonadotropin\u2010releasing hormone\u2010I (GnRH\u2010I) mRNA expression in the preoptic hypothalamus was observed in 0.5 and 5\u2009\u00b5g MUSC\u2010treated fish compared to experimental controls; however, a significant upregulation of luteinizing hormone\u2010\u03b2 (LH\u2010\u03b2) mRNA expression was observed only at the highest dose. A similar stimulatory effect on GnRH\u2010I mRNA expression was observed in the 5, 10, or 50\u2009\u00b5g BAC\u2010treated group compared to experimental controls, but no significant change was observed in LH\u2010\u03b2 mRNA level. Furthermore, treatment with 0.5 or 5\u2009\u00b5g MUSC stimulated the development of all stages of follicles in the ovary except for Stages IV or III, respectively. On the other hand, treatment with 50\u2009\u00b5g BAC caused a significant increase in the numbers of Stages I\u2013V follicles. These results were concomitant with a significant increase in some of the embryonic developmental stages and fecundity levels in 0.5 or 5\u2009\u00b5g MUSC\u2010treated or 50\u2009\u00b5g BAC\u2010treated fish compared to those of experimental controls. Together, these findings suggest that GABA<jats:sub>A<\/jats:sub> and GABA<jats:sub>B<\/jats:sub> receptor agonists produce a stimulatory effect on the HPO axis in a receptor\u2010specific and concentration\u2010dependent manner for the first time in a viviparous teleost.<\/jats:p>","DOI":"10.1002\/mrd.70041","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T08:35:40Z","timestamp":1752741340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["GABA<sub>A<\/sub> and GABA<sub>B<\/sub> Receptor Agonists Promote the Hypothalamic\u2013Pituitary\u2013Ovarian Axis in the Postfertilization Model <i>Poecilia sphenops<\/i>"],"prefix":"10.1002","volume":"92","author":[{"given":"Achyutham","family":"Hotha","sequence":"first","affiliation":[{"name":"Neuroendocrinology Research Laboratory, Department of Studies in Zoology Karnatak University Dharwad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5662-534X","authenticated-orcid":false,"given":"C. 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