{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T12:10:09Z","timestamp":1759061409099,"version":"3.44.0"},"reference-count":62,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Circular RNAs are associated with important pathophysiological characteristics of gestational diabetes mellitus (GDM). This study preliminarily explored circular polyribonucleotide nucleotidyltransferase 1 (circ\u2010PNPT1) expression in placental tissues and serum of GDM patients and its role in regulating the miR\u2010144\u20103p\/UBE2G1 axis to affect endothelial dysfunction in GDM. Twenty GDM patients and 20 pregnant women with normal glucose tolerance were enrolled. Human umbilical vein endothelial cells (HUVECs) were cultured with high glucose (HG) to establish an in vitro GDM model, then treated with short hairpin\u2010circ\u2010PNPT1 and a lentiviral empty plasmid. Circ\u2010PNPT1 levels, HUVEC viability, endothelial function, and oxidative damage were assessed using RT\u2010qPCR tube formation, CCK\u20108, flow cytometry, and Transwell assays, respectively. Normally cultured and HG\u2010cultured HUVEC samples underwent small RNA sequencing to analyze differentially expressed miRNAs. The possible miRNAs and mRNAs downstream of circ\u2010PNPT1 were screened using bioinformatics and verified using RT\u2010qPCR, dual luciferase reporter assay, and Ago2\u2010RIP. GDM patients exhibited highly expressed circ\u2010PNPT1 and UBE2G1, and weakly expressed miR\u2010144\u20103p in placental tissues and serum. In vitro, HG\u2010treated HUVECs displayed highly expressed circ\u2010PNPT1 and cellular dysfunction, as evidenced by reduced cell survival, enhanced apoptosis, decreased cell migration and angiogenesis, an elevated MDA level, and downregulated SOD and GSH\u2010Px levels. Circ\u2010PNPT1 knockdown alleviated HG\u2010induced HUVEC dysfunction. circ\u2010PNPT1 might target and bind miR\u2010144\u20103p; miR\u2010144\u20103p might target and bind UBE2G1. Additionally, circ\u2010PNPT1 might act as a competing endogenous RNA for miR\u2010144\u20103p to regulate UBE2G1 expression in HG\u2010induced HUVECs. Taken together, circ\u2010PNPT1 modulates HG\u2010induced HUVEC dysfunction via the miR\u2010144\u20103p\/UBE2G1 axis.<\/jats:p>","DOI":"10.1002\/mrd.70057","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T14:44:56Z","timestamp":1758811496000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Preliminary Study of circ\u2010PNPT1\/miR\u2010144\u20103p\/UBE2G1 Expression in Placental Tissues and Serum and Its Mechanism in Patients With Gestational Diabetes Mellitus"],"prefix":"10.1002","volume":"92","author":[{"given":"Jingyi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Center of Maternal\u2010Fetal Medicine The First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minire","family":"Tuluhong","sequence":"additional","affiliation":[{"name":"Department 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