{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:23:39Z","timestamp":1790882619283,"version":"4.1.0"},"reference-count":77,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Dairy cows often experience a period of negative energy balance (NEB) during the post\u2010calving period, which can significantly impact economic outcomes due to extended calving\u2010to\u2010conception intervals and overall reduced fertility. This reduction is due, in part, to the impact on uterine biology by high nonesterified fatty acids (NEFA) and beta\u2010hydroxybutyrate concentration. The uterine fluid (UF) contains small extracellular vesicles (UF\u2010EVs) that, through their cargo, including microRNAs (miRNAs), respond to metabolic stress, affecting the uterine environment. This study aimed to assess the long\u2010term impact of NEB intensity on the uterine environment of dairy cows. Post\u2010partum dairy cows were classified based on NEFA concentrations in their blood during the 3 weeks post\u2010calving as having either Low or High NEB. At 30 and 60 DPC, the synchronization protocol was started, and UF samples were collected (corresponding to ~15 days after initiation of the synchronization protocol) to isolate UF\u2010EVs and uterine epithelial cells for miRNA and transcriptome profiling. We also investigated whether UF\u2010EVs could modulate epithelial uterine na\u00efve cells. Our results indicate that the uterine environment of dairy cows experiencing a High NEB post\u2010calving is unfavorable for embryo development at 60\u2010day post\u2010calving. Importantly, we show that UF\u2010EVs can reproduce this phenotype in epithelial uterine na\u00efve cells, suggesting that UF\u2010EVs may act as modulators of the uterine response to metabolic challenges.<\/jats:p>","DOI":"10.1002\/mrd.70062","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:32:51Z","timestamp":1761219171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Uterine Extracellular Vesicles Can Emulate the Long\u2010Term Effects of Post\u2010Partum Negative Energy Balance in Dairy Cows"],"prefix":"10.1002","volume":"92","author":[{"given":"Juliana Germano","family":"Ferst","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering University of S\u00e3o Paulo  Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus 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