{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T05:19:13Z","timestamp":1766985553519,"version":"3.48.0"},"reference-count":48,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The search for male\u2010biased genes that may drive sexual differentiation in sturgeon gonadal tissue has remained largely fruitless until now. This study explored the presence, expression, and sexual steroid regulation of gonadal soma\u2010derived factor (Gsdf) in Siberian sturgeon (\n                    <jats:italic>Acipenser baerii<\/jats:italic>\n                    ). Three sequences compatible with the protein\u2010coding gene\n                    <jats:italic>gsdf<\/jats:italic>\n                    were identified in a Siberian sturgeon gonadal transcriptome database. The phylogenetic analysis shows the presence of two paralogues\n                    <jats:italic>gsdfa<\/jats:italic>\n                    and\n                    <jats:italic>gsdfb<\/jats:italic>\n                    , and two clearly identifiable\n                    <jats:italic>gsdfa<\/jats:italic>\n                    isoforms (\n                    <jats:italic>gsdfa1<\/jats:italic>\n                    and\n                    <jats:italic>gsdfa2<\/jats:italic>\n                    ).\n                    <jats:italic>gsdfa<\/jats:italic>\n                    was expressed in sex\u2010undifferentiated gonads, brain, hypophysis, heart, interrenal, intestine, liver, stomach, embryos and larvae, while\n                    <jats:italic>gsdfb<\/jats:italic>\n                    was specific to the gonads.\n                    <jats:italic>gsdfb<\/jats:italic>\n                    showed significantly higher expression in genetically sexed males during late stage of sex differentiation. In contrast,\n                    <jats:italic>gsdfa1<\/jats:italic>\n                    and\n                    <jats:italic>gsdfa2<\/jats:italic>\n                    showed similar gonadal expression levels in both sexes. In terms of sensitivity to sexual steroids, only\n                    <jats:italic>gsdfb<\/jats:italic>\n                    was repressed significantly and in a dose\u2010dependent manner by estrogens. Taken together, these results indicate both that\n                    <jats:italic>gsdfb<\/jats:italic>\n                    is part of the male gonadal differentiation pathway in sturgeon and that its repression by estrogens is fundamental for female development.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70074","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T05:56:03Z","timestamp":1765259763000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>gsdfb<\/i>\n                    , But Not the Isoforms\n                    <i>gsdfa1<\/i>\n                    and\n                    <i>gsdfa2<\/i>\n                    , Is Up\u2010Regulated During the Male Program and Repressed by Estrogens in Siberian Sturgeon"],"prefix":"10.1002","volume":"92","author":[{"given":"German","family":"Benech\u2010Correa","sequence":"first","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda de la Reproducci\u00f3n y Ecolog\u00eda de Peces, Instituto de Biolog\u00eda, Facultad de Ciencias Universidad de la Rep\u00fablica Oriental del Uruguay Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9","family":"Lasalle","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda de la Reproducci\u00f3n y Ecolog\u00eda de Peces, Instituto de Biolog\u00eda, Facultad de Ciencias Universidad de la Rep\u00fablica Oriental del Uruguay Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9deric","family":"Brunet","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9nomique Fonctionnelle de Lyon, UMR5242, Ecole Normale Sup\u00e9rieure de Lyon, Centre National de la Recherche Scientifique Universit\u00e9 Claude Bernard\u2010Lyon 1 Lyon France France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7529-7556","authenticated-orcid":false,"given":"Denise","family":"Vizziano\u2010Cantonnet","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda de la Reproducci\u00f3n y Ecolog\u00eda de Peces, Instituto de Biolog\u00eda, Facultad de Ciencias Universidad de la Rep\u00fablica Oriental del Uruguay Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.041830"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-012-9666-5"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evy101"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62408-x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-023-05245-9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep19480"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2013.06.010"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1049"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2010.04.011"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056006"},{"volume-title":"Techniques 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