{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T10:50:24Z","timestamp":1791111024419,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["407992\/2021\u20109"],"award-info":[{"award-number":["407992\/2021\u20109"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005283","name":"Funda\u00e7\u00e3o Cearense de Apoio ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["BMD_0008_00273.01.13\/21"],"award-info":[{"award-number":["BMD_0008_00273.01.13\/21"]}],"id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Oocyte in vitro maturation (IVM) represents a crucial phase in embryo production, often compromised by reactive oxygen species (ROS). Strategies to mitigate ROS are essential to improve oocyte quality. This study investigated the effects of liposome\u2010encapsulated \u03b1\u2010pinene (Lip\u2010\u03b1\u2010pinene) on bovine oocyte IVM and embryonic development following parthenogenetic activation. Cumulus\u2010oocyte complexes (COCs) were matured in vitro for 22\u201324\u2009h in control medium or supplemented with Lip\u2010blank, or 0.01, 1.0 or 100.0\u2009\u00b5g\/mL Lip\u2010\u03b1\u2010pinene. Lip\u2010\u03b1\u2010pinene had a size of 75.86\u2009\u00b1\u20090.95\u2009nm, low polydispersity (0.26\u2009\u00b1\u20090.00), and zeta potential of \u221231.55\u2009\u00b1\u20092.23\u2009mV. Nuclear maturation was not affected. However, COCs matured with 1.0\u2009\u00b5g\/mL Lip\u2010\u03b1\u2010pinene showed well\u2010preserved ultrastructure of oocyte and cumulus cells, reduced ROS levels and lipid accumulation compared to control, Lip\u2010blank, and 100.0\u2009\u00b5g\/mL Lip\u2010\u03b1\u2010pinene. This was accompanied by relative abundance of\n                    <jats:italic>NRF2<\/jats:italic>\n                    ,\n                    <jats:italic>SOD<\/jats:italic>\n                    , and\n                    <jats:italic>PRDX6<\/jats:italic>\n                    . Furthermore, 1.0 and 100.0\u2009\u00b5g\/mL Lip\u2010\u03b1\u2010pinene increased cleavage rates and cells per blastocyst compared to control, while blastocyst rates and lipid content were not affected. In conclusion, 1.0\u2009\u00b5g\/mL Lip\u2010\u03b1\u2010pinene enhances antioxidant capacity of bovine oocytes by reducing ROS and lipid accumulation, associated with abundance of\n                    <jats:italic>NRF2<\/jats:italic>\n                    ,\n                    <jats:italic>SOD<\/jats:italic>\n                    , and\n                    <jats:italic>PRDX6<\/jats:italic>\n                    transcripts, thereby improving oocyte competence and quality of parthenogenetic embryos.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70094","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T12:09:17Z","timestamp":1771502957000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of Liposome\u2010Encapsulated \u03b1\u2010Pinene on In Vitro Oocyte Maturation and Embryo Development in Bovine Species"],"prefix":"10.1002","volume":"93","author":[{"given":"Ven\u00e2ncia Antonia Nunes","family":"Azevedo","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology and Physiology of Reproduction Federal University of Ceara Sobral Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Alice de","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus de Andrade","family":"Chaves","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca Angi","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiziana Cavalcante Costa Fernandes","family":"Cris\u00f3stomo","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Odontology and Nursing Federal University of Ceara Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Arag\u00e3o Matos","family":"Donato","sequence":"additional","affiliation":[{"name":"Laboratory of Ultrastructure, CPqAM\/FIOCRUZ Federal University of Pernambuco Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina Alves","family":"Peixoto","sequence":"additional","affiliation":[{"name":"Laboratory of Ultrastructure, CPqAM\/FIOCRUZ Federal University of Pernambuco Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josimar Oliveira","family":"Eloy","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Odontology and Nursing Federal University of Ceara Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1vio Vieira","family":"Meirelles","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Perecin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4796-6393","authenticated-orcid":false,"given":"Juliano Coelho da","family":"Silveira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5970-6177","authenticated-orcid":false,"given":"Jos\u00e9 Roberto Viana","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Physiology of Reproduction Federal University of Ceara Sobral Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1131"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.10.017"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2021.112857"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22316"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina61122107"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-2836(65)80093-6"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1060160405"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2021.121086"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2020.100700"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29030607"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abq4835"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1071\/rd15067"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-17-0134"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep-13-0251"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf901162f"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2016.01.032"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2020.111185"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0284809"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2017.08.008"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.62310\/liab.v4i2.145"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26226840"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep-10-0045"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c07339"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2018.10.004"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14020330"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.11.017"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13050597"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaq007"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20824"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.11.014"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14050910"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.12.058"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1017\/s0967199422000673"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.5003726"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.11.028"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2022.e09394"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12275"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2020.03.016"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2014.950672"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1515\/znc-2014-4135"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2016.05.016"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.007799"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.05.026"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-020-00190-x"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23630"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2023.106824"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2022.08.024"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1071\/rd10339"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.4103\/apjr.apjr_110_24"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1658508"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28031498"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.09.012"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10010127"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1590\/fst.46521"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolreprod\/7.3.425"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.70094","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mrd.70094","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.70094","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T04:33:55Z","timestamp":1773030835000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.70094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":56,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/mrd.70094"],"URL":"https:\/\/doi.org\/10.1002\/mrd.70094","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"2025-09-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70094"}}