{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T14:12:04Z","timestamp":1779891124039,"version":"3.53.1"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32161143032"],"award-info":[{"award-number":["32161143032"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In vitro production (IVP) of bovine embryos often results in lower developmental competence compared to in vivo\u2010derived embryos. This disparity is further exacerbated by oocyte cryopreservation (vitrification). This study aimed to investigate the molecular basis of reduced embryo quality by profiling the transcriptomic impact of in vitro maturation (IVM) and vitrification on bovine blastocysts.Day 7 blastocysts from three groups were analyzed: (1) in vivo: control embryos produced entirely in vivo, (2) IVF: embryos from fresh oocytes subjected to IVM and in vitro fertilization, and (3) V_IVF: embryos from vitrified\u2010thawed oocytes subjected to IVM and fertilization. Whole\u2010blastocyst transcriptome profiling was performed using single\u2010embryo RNA sequencing (SUPeR\u2010seq;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093 blastocysts per group) and analyzed without cellular dissociation. Developmental competence was significantly reduced in the V_IVF group (~\u200911.8% blastocyst rate) compared to the IVF group (~\u200941.5%) and in vivo controls. Transcriptome analysis revealed 362 differentially expressed mRNAs in V_IVF embryos and 78 in IVF embryos compared to in vivo controls. Genes such as\n                    <jats:italic>ATP1A1, GOT1L1<\/jats:italic>\n                    , and\n                    <jats:italic>QSOX1<\/jats:italic>\n                    were significantly altered, influencing pathways related to oxidative phosphorylation, lysosome activity, and chromatin organization. In addition, 694 DE\u2010lncRNAs in the V_IVF group and 187 in the IVF group were identified. Exonic circRNAs (608) were detected with differential expression and enrichment in signaling and epigenetic pathways, including Hedgehog signaling and stem cell pluripotency regulation. Integrated network analysis revealed coordinated dysregulation across mRNA, lncRNA, and circRNA layers, suggesting disruption of multi\u2010layered regulatory circuits. Overall, this study presents a detailed transcriptomic landscape of bovine blastocysts affected by IVM and vitrification.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70116","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T05:27:02Z","timestamp":1779859622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Embryo RNA Sequencing Reveals Transcriptomic Alterations in Blastocysts Derived From IVM or Vitrified Oocytes"],"prefix":"10.1002","volume":"93","author":[{"given":"Hubdar Ali","family":"Kolachi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Institute of Animal Husbandry and Veterinary Sciences Xinjiang Academy of Agricultural and Reclamation Sciences Shihezi China"},{"name":"Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sindh Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Pei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Tianjin Academy of Agricultural Sciences Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4910-151X","authenticated-orcid":false,"given":"Jesse Oluwaseun","family":"Ayantoye","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS) Chinese Academy of Agricultural Sciences (CAAS) No.2 Yuanmingyuan West Road Haidian District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Shahzad","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS) Chinese Academy of Agricultural Sciences (CAAS) No.2 Yuanmingyuan West Road Haidian District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baigao","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS) Chinese Academy of Agricultural Sciences (CAAS) No.2 Yuanmingyuan West Road Haidian District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS) Chinese Academy of Agricultural Sciences (CAAS) No.2 Yuanmingyuan West Road Haidian District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS) Chinese Academy of Agricultural Sciences (CAAS) No.2 Yuanmingyuan West Road Haidian District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyi","family":"Feng","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences (IAS) Chinese Academy of Agricultural Sciences (CAAS) No.2 Yuanmingyuan West Road Haidian District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Ibrahim","family":"Panhwar","sequence":"additional","affiliation":[{"name":"Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sindh Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengcheng","family":"Wan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Institute of Animal Husbandry and Veterinary Sciences Xinjiang Academy of Agricultural and Reclamation Sciences Shihezi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueming","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Institute of Animal Husbandry and Veterinary Sciences Xinjiang Academy of Agricultural and Reclamation Sciences Shihezi China"},{"name":"Institute of Animal Sciences (IAS) Chinese Academy of Agricultural Sciences (CAAS) No.2 Yuanmingyuan West Road Haidian District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s40104-021-00672-1","article-title":"Unveiling How Vitrification Affects the Porcine Blastocyst: Clues From a Transcriptomic Study","volume":"13","author":"Almi\u00f1ana C.","year":"2022","journal-title":"Journal of Animal Science and Biotechnology"},{"issue":"2","key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.theriogenology.2007.05.044","article-title":"Expression Pattern of Oxygen and Stress\u2010Responsive Gene Transcripts at Various Developmental Stages of In Vitro and In Vivo Preimplantation Bovine 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