{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:42:57Z","timestamp":1792082577860,"version":"4.4.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Diminished ovarian reserve (DOR) is a major cause of female infertility. In recent years, traditional Chinese medicine has displayed unique ameliorating effects on DOR. This study aims to investigate the therapeutic efficacy and molecular mechanisms of Bushen Zhuluan Decoction (BSZLD) in DOR. A rat model of DOR was established and treated with either BSZLD or Progynova (positive control). Estrous cycle monitoring, ovarian index calculation, hematoxylin\u2010eosin staining, and enzyme\u2010linked immunosorbent assay were performed to assess ovarian reserve function. TUNEL staining, immunohistochemistry, and Western blotting were conducted to evaluate ovarian cell apoptosis. To elucidate the pharmacological basis of BSZLD, ultra\u2010high\u2010performance liquid chromatography\u2010tandem mass spectrometry was applied. Network pharmacology analysis, machine learning algorithms, and molecular docking were integrated to predict key active compounds and pathways. The role of the sirtuin 1 (SIRT1) and its downstream target hypoxia\u2010inducible factor 1alpha (HIF\u20101\u03b1) signaling in BSZLD\u2010mediated effects was verified using short hairpin RNA (shRNA) lentiviral\u2010mediated interference. BSZLD significantly restored estrous cycle disturbances, increased ovarian index and follicle counts, and reduced atretic follicles and ovarian cell apoptosis in DOR rats. Serum anti\u2010M\u00fcllerian hormone and estradiol levels were elevated, while follicle\u2010stimulating hormone and luteinizing hormone levels were reduced. The SIRT1\/HIF\u20101\u03b1 signaling pathway was a core regulatory axis for BSZLD action. BSZLD markedly upregulated SIRT1 expression while suppressing HIF\u20101\u03b1 expression. Silencing SIRT1 using shRNA reduced SIRT1 levels, elevated HIF\u20101\u03b1 expression, and significantly attenuated the therapeutic effects of BSZLD. BSZLD attenuates DOR in rats by activating the SIRT1\/HIF\u20101\u03b1 signaling, thereby suppressing ovarian cell apoptosis and restoring ovarian reserve function.<\/jats:p>","DOI":"10.1002\/mrd.70117","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T11:20:44Z","timestamp":1779794444000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bushen Zhuluan Decoction Ameliorates Diminished Ovarian Reserve in Rats via the SIRT1\/HIF\u20101\u03b1 Pathway"],"prefix":"10.1002","volume":"93","author":[{"given":"Chuqiang","family":"Shu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, NHC Key Laboratory of Birth Defect for Research and Prevention Hunan Provincial Maternal and Child Health Care Hospital Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6190-0547","authenticated-orcid":false,"given":"Huizhen","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine, NHC Key Laboratory of Birth Defect for Research and Prevention Hunan Provincial Maternal and Child Health Care Hospital Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhizhen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine, NHC Key Laboratory of Birth Defect for Research and Prevention Hunan Provincial Maternal and Child Health Care Hospital Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine, NHC Key Laboratory 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of Hunan University of Chinese Medicine Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Xia","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Hunan University of Chinese Medicine Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-024-01427-y"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.162474"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2020.111215"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-025-01643-0"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.27138\/d.cnki.ghuzc.2023.000365"},{"issue":"11","key":"e_1_2_10_7_1","first-page":"1683","article-title":"Upregulation of PLGF and Flt\u20101 Levels in 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