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This study investigated this issue in cloned pigs at the 35th day of gestation, where a 69.8% developmental abnormality rate was observed. Morphological analyses revealed structural defects in placentas from failed pregnancies, including delayed fold development and abnormal areolae formation, coupled with reduced endometrial glands and maternal\u2010side blood vessels. Immunohistochemistry demonstrated impaired placental function, evidenced by aberrant E\u2010cadherin expression compromising trophoblast adhesion, and significantly reduced glycogen\/polysaccharide secretion. Furthermore, Plet1 expression was disrupted. Transcriptomic profiling indicated that upregulated genes were enriched in cell\u2010cell tight junctions, while downregulated genes were associated with oxygen binding, oxidative stress, stromal differentiation, and vascular development. Cross\u2010species comparison with cloned mice suggested conserved genetic mechanisms underlying placental defects. These findings provide a crucial theoretical foundation for elucidating the mechanisms of placental failure in animal cloning and for developing strategies to enhance its efficiency.<\/jats:p>","DOI":"10.1002\/mrd.70122","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T10:48:41Z","timestamp":1781520521000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Placental Dysfunction in Cloned Pigs Linked to Impaired Trophoblast Adhesion and Vascular Development"],"prefix":"10.1002","volume":"93","author":[{"given":"Xia","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine Huazhong Agricultural University Wuhan China"},{"name":"Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction (Huazhong Agricultural University), Ministry of Education Wuhan 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