{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:00:51Z","timestamp":1790017251740,"version":"4.0.1"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Early embryonic mortality is a major concern to the beef industry, as nearly 32.3% of multiparous cows will not maintain pregnancy past the first sixteen days of gestation. During this period, the uterine environment provides nutrients to support embryonic growth and development. Therefore, our objective was to (1) modulate the luminal metabolome at the onset of conceptus elongation in pregnant beef cows and (2) identify changes in the endometrial epithelial transcriptome during the peri\u2010elongation period. Cows received either TARG: 100\u2009g of a rumen\u2010inert mono\u2010 and polyunsaturated fatty acid source plus 60\u2009g of a rumen\u2010protected choline source (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200950) or CON: 114\u2009g of a saturated fatty acid source (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200950) daily from 30 days before (d \u221230) to 30 days after breeding (d 30). Uterine cytobrush was collected from pregnant cows on day 14 for metabolomics (CON\u2009=\u200917, TARG\u2009=\u200915) and on Day 16 for transcriptomics analysis (CON\u2009=\u20096, TARG\u2009=\u20097). On Day 14, the concentration of 23 metabolites (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.1) differed; the concentration of fatty acids, triacylglycerols, hexosylceramides, cholesterol esters, phospholipids, biogenic amines, carboxylic acids, methyl donors, and amino acids was greater in TARG. On Day 16, 675 genes were differentially expressed (FDR\u2009\u2264\u20090.1). Among these genes, 506 genes were upregulated in TARG, involving important genes in pathways such as interferon signaling, fatty acid transport, synthesis, metabolism, immune response, and cell\u2010to\u2010cell adhesion. In conclusion, the dietary supplementation provided herein modulated the uterine environment during the period of conceptus elongation.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70131","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T07:17:43Z","timestamp":1783581463000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary Supplementation of Rumen\u2010Inert Fatty Acids and Rumen\u2010Protected Choline Modulates the Uterine Environment of Pregnant Beef Cows"],"prefix":"10.1002","volume":"93","author":[{"given":"Megan","family":"Bahr","sequence":"first","affiliation":[{"name":"Department of Animal Science North Carolina State University Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allyson","family":"McCarty","sequence":"additional","affiliation":[{"name":"Department of Animal Science North Carolina State University Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaeem","family":"Sarwar","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences University of Florida Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damon","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Animal Science Texas A&amp;M University College Station Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Poore","sequence":"additional","affiliation":[{"name":"Department of Animal Science North Carolina State University Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3625-4419","authenticated-orcid":false,"given":"Daniel","family":"Poole","sequence":"additional","affiliation":[{"name":"Department of Animal Science North Carolina State University Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Ashrafi","sequence":"additional","affiliation":[{"name":"Metabolomics Department Corewell Health Research Institute Royal Oak Michigan USA"},{"name":"William Beaumont University Hospital Royal Oak Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romana","family":"Mimi","sequence":"additional","affiliation":[{"name":"Metabolomics Department Corewell Health Research Institute Royal Oak Michigan USA"},{"name":"William Beaumont University Hospital Royal Oak Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart","family":"Graham","sequence":"additional","affiliation":[{"name":"Metabolomics Department Corewell Health Research Institute Royal Oak Michigan USA"},{"name":"William Beaumont University Hospital Royal Oak Michigan USA"},{"name":"Oakland University\u2010William Beaumont School of Medicine Rochester Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ky","family":"Pohler","sequence":"additional","affiliation":[{"name":"Department of Animal Science Texas A&amp;M University College Station Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences University of Florida Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Maltecca","sequence":"additional","affiliation":[{"name":"Department of Animal Science North Carolina State University Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9189-0912","authenticated-orcid":false,"given":"Felipe A. 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