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In Experiment 1, embryos were exposed to hypothermia for either 6 or 12\u2009h on day 5 or 6 of development, and the blastocyst rates and kinetics were evaluated. In Experiment 2, the transcriptome of embryos exposed to hypothermia on either day 5 or 6 for 12\u2009h was assessed. In Experiment 3, embryos were exposed to hypothermia for 12\u2009h on day 6, vitrified\u2010warmed, and their re\u2010expansion, apoptotic index, and total cell number were assessed. Results showed that hypothermia had no effect on blastocyst rate but reduced the percentage of hatched blastocysts when applied for 12\u2009h. Moreover, hypothermia was associated with changes in the transcriptional profile, particularly in embryos exposed on day 6, including increased expression of the cold\u2010shock\u2010related transcripts\n                    <jats:italic>RBM3<\/jats:italic>\n                    and\n                    <jats:italic>CIRBP<\/jats:italic>\n                    . Finally, hypothermia on day 6 did not improve embryo re\u2010expansion after cryopreservation but did reduce apoptosis in the surviving embryos. Our results provide insight into transcriptomic responses associated with mild hypothermia in bovine embryos and support the hypothesis that hormetic\u2010like responses may contribute to improved embryo quality after cryopreservation.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70133","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T07:29:33Z","timestamp":1783322973000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Sublethal Mild Hypothermia on Kinetics, Transcriptome, and Cryotolerance of\n                    <i>In Vitro<\/i>\n                    Produced Bovine Embryos"],"prefix":"10.1002","volume":"93","author":[{"given":"Luciano C.","family":"Pantoja","sequence":"first","affiliation":[{"name":"Veterinary Medicine Institute Federal University of Para Castanhal Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio A.","family":"Garcia Pereira\u2010Junior","sequence":"additional","affiliation":[{"name":"Department of Genetics and Evolution Federal University of S\u00e3o Carlos S\u00e3o Carlos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izamara S. 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